| Autor: |
Vargas-Casanova Y; Microbiology Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia., Bravo-Chaucanés CP; Microbiology Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia., Martínez AXH; Chemistry Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia., Costa GM; Chemistry Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia., Contreras-Herrera JL; Faculty of Engineering and Basic Sciences, Insitituto Tecnológico del Putumayo, Mocoa 860001, Colombia., Medina RF; Faculty of Sciences, Universidad Nacional of Colombia, Bogotá 111321, Colombia., Rivera-Monroy ZJ; Faculty of Sciences, Universidad Nacional of Colombia, Bogotá 111321, Colombia., García-Castañeda JE; Faculty of Sciences, Universidad Nacional of Colombia, Bogotá 111321, Colombia., Parra-Giraldo CM; Microbiology Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia. |
| Abstrakt: |
The antifungal activity of palindromic peptide RWQWRWQWR and its derivatives was evaluated against clinical isolates of Candida albicans and C. auris . Also, Bidens pilosa ethanolic extracts of leaves and stem were evaluated. Furthermore, combinations of peptide, extract, and/or fluconazole (FLC) were evaluated. The cytotoxicity of peptides and extracts in erythrocytes and fibroblasts was determined. The original palindromic peptide, some derivative peptides, and the ethanolic extract of leaves of B. pilosa exhibited the highest activity in some of the strains evaluated. Synergy was obtained between the peptide and the FLC against C. auris 435. The combination of the extract and the original palindromic peptide against C. albicans SC5314, C. auris 435, and C. auris 537 decreased the minimal inhibitory concentrations (MICs) by a factor of between 4 and 16. These mixtures induced changes in cell morphology, such as deformations on the cell surface. The results suggest that the combination of RWQWRWQWR and B. pilosa extract is an alternative for enhancing antifungal activity and decreasing cytotoxicity and costs and should be considered to be a promising strategy for treating diseases caused by Candida spp. |
