Does dinocyst wall composition really reflect trophic affinity? New evidence from ATR micro-FTIR spectroscopy measurements.

Autor: Meyvisch P; Department of Geology, Ghent University, Ghent, Belgium., Mertens KN; Ifremer, LITTORAL, France., Gurdebeke PR; Department of Geology, Ghent University, Ghent, Belgium., Sandt C; SMIS Beamline, SOLEIL Synchrotron, Paris, France., Pospelova V; Department of Earth and Environmental Sciences, University of Minnesota, Minneapolis, Minnesota, USA., Vrielinck H; Department of Solid-State Sciences, Ghent University, Ghent, Belgium., Borondics F; SMIS Beamline, SOLEIL Synchrotron, Paris, France., Louwye S; Department of Geology, Ghent University, Ghent, Belgium.
Jazyk: angličtina
Zdroj: Journal of phycology [J Phycol] 2023 Oct; Vol. 59 (5), pp. 1064-1084. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1111/jpy.13382
Abstrakt: Attenuated total reflection (ATR) microscope Fourier transform infrared (micro-FTIR) spectroscopy was used to investigate the dinosporin composition in the walls of modern, organic-walled dinoflagellate resting cysts (dinocysts). Variable cyst wall compositions were observed, which led to the erection of four spectrochemical groups, some with striking similarities to other resistant biomacromolecules such as sporopollenin and algaenan. Furthermore, possible proxies derivable from the spectrochemical composition of modern and fossil dinocysts were discussed. The color of the dinocyst walls was reflected in the spectral data. When comparing that color with a standard and the results of a series of bleaching experiments with oxidative agents, eumelanin was assigned as a likely pigment contributing to the observed color. Following this assignment, the role of eumelanin as an ultraviolet sunscreen in colored dinocysts was hypothesized, and its implications on the autofluorescence and morphological preservation of dinocysts were further discussed. Unlike what had previously been assumed, it was shown that micro-FTIR data from dinocysts cannot be used to unambiguously infer trophic affinities of their associated cells. Finally, using methods with high spatial resolutions (synchrotron transmission micro-FTIR and optical photothermal infrared spectroscopy), it was shown that dinocyst wall layers are chemically homogenous at the probed scales. This study fills a large knowledge gap in our understanding of the chemical nature of dinocyst walls and has nuanced certain assumptions and interpretations made in the past.
(© 2023 Phycological Society of America.)
Databáze: MEDLINE
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