openFrame: A modular, sustainable, open microscopy platform with single-shot, dual-axis optical autofocus module providing high precision and long range of operation.

Autor: Lightley J; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK., Kumar S; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK., Lim MQ; Photonics Group, Physics Department, Imperial College London, London, UK.; Department of Surgery and Cancer, Imperial College London, London, UK., Garcia E; Photonics Group, Physics Department, Imperial College London, London, UK.; Department of Surgery and Cancer, Imperial College London, London, UK., Görlitz F; Photonics Group, Physics Department, Imperial College London, London, UK., Alexandrov Y; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK., Parrado T; Cairn Research Ltd, Faversham, Kent, England., Hollick C; Cairn Research Ltd, Faversham, Kent, England., Steele E; Cairn Research Ltd, Faversham, Kent, England., Roßmann K; Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany., Graham J; Cairn Research Ltd, Faversham, Kent, England., Broichhagen J; Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany., McNeish IA; Department of Surgery and Cancer, Imperial College London, London, UK., Roufosse CA; Department of Inflammation and Immunology, Imperial College London, London, UK., Neil MAA; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK., Dunsby C; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK., French PMW; Photonics Group, Physics Department, Imperial College London, London, UK.; Francis Crick Institute, London, UK.
Jazyk: angličtina
Zdroj: Journal of microscopy [J Microsc] 2023 Nov; Vol. 292 (2), pp. 64-77. Date of Electronic Publication: 2023 Sep 27.
DOI: 10.1111/jmi.13219
Abstrakt: 'openFrame' is a modular, low-cost, open-hardware microscopy platform that can be configured or adapted to most light microscopy techniques and is easily upgradeable or expandable to multiple modalities. The ability to freely mix and interchange both open-source and proprietary hardware components or software enables low-cost, yet research-grade instruments to be assembled and maintained. It also enables rapid prototyping of advanced or novel microscope systems. For long-term time-lapse image data acquisition, slide-scanning or high content analysis, we have developed a novel optical autofocus incorporating orthogonal cylindrical optics to provide robust single-shot closed-loop focus lock, which we have demonstrated to accommodate defocus up to ±37 μm with <200 nm accuracy, and a two-step autofocus mode which we have shown can operate with defocus up to ±68 μm. We have used this to implement automated single molecule localisation microscopy (SMLM) in a relatively low-cost openFrame-based instrument using multimode diode lasers for excitation and cooled CMOS cameras.
(© 2023 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.)
Databáze: MEDLINE
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