Taxogenomics of Alkalihalobacterium chitinilyticum sp. nov.: an alkaliphilic chitin degrading bacterial strain isolated from Lonar Lake, India, with potential biotechnological applications.

Autor: Thite S; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India., Tarwadge K; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India., Mengade P; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India., Lodha T; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India.; Bioenergy Group, Agharkar Research Institute, Gopal Ganesh Agarkar Road, Pune, Maharashtra, 411004, India., Joseph N; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India., Thakkar L; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India., Joshi A; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India. amaraja@nccs.res.in.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2023 Nov; Vol. 116 (11), pp. 1103-1112. Date of Electronic Publication: 2023 Aug 24.
DOI: 10.1007/s10482-023-01872-7
Abstrakt: A novel chitin degrading alkaliphilic bacterial strain (MEB 203  T ) was isolated from sediment collected from Lonar lake, India. The strain exhibited its maximum growth at a temperature of 37 °C, with an optimal pH of 10 and a NaCl concentration of 2%. 16S rRNA gene based phylogenetic tree showed that strain was closely related to Alkalihalobacterium elongatum MCC 2982  T (98.64% similarity) followed by A. alkalinitrilicum DSM 22532  T (97.84% similarity). The genome size was 4.9 Mb with DNA G + C content of 37.7%. The dDDH value between strain MEB 203  T and A. elongatum MCC 2982  T was 26.4 ± 2.4% while OrthoANI value was 82.1%. Genome analysis revealed the presence of genes responsible for L-ectoine and cation/proton antiporter which may facilitate growth of strain in alkaline-saline habitat of Lonar lake. Strain MEB 203  T was able to utilize complex sugars such as chitin, cellulose, and starch as a carbon source at alkaline conditions which was also corroborated from the genomic presence of carbohydrate active enzymes (CAZymes). It was also able to produce biotechnologically important enzymes such as lipases and proteases which were stable at pH (9-10). The bacterium is majorly composed of C 15:0 iso, C 16:0 iso, and C 17:0 iso (> 10%) fatty acids while diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified phospholipid (PL3) were identified as the predominant polar lipids. Based on differential physiological, biochemical, and genomic features of strain MEB 203  T , a novel species Alkalihalobacterium chitinilyticum sp. nov. (Type strain MEB 203  T  = MCC 3920  T  = NCIMB 15407  T  = JCM 35078  T ) is proposed.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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