Reliability of the Enterprise Point-of-Care (EPOC) blood analyzer's calculated arterial oxygen-hemoglobin saturation in immobilized white rhinoceroses (Ceratotherium simum).

Autor: Mtetwa TK; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Centre for Veterinary Wildlife Research, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Snelling EP; Centre for Veterinary Wildlife Research, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Donaldson AC; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Centre for Veterinary Wildlife Research, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Buss PE; Centre for Veterinary Wildlife Research, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Veterinary Wildlife Services, South African National Parks, Kruger National Park, Skukuza, South Africa.; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Meyer LCR; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.; Centre for Veterinary Wildlife Research, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Jazyk: angličtina
Zdroj: Veterinary clinical pathology [Vet Clin Pathol] 2023 Sep; Vol. 52 (3), pp. 417-421. Date of Electronic Publication: 2023 Aug 23.
DOI: 10.1111/vcp.13236
Abstrakt: Background: Enterprise Point-of-Care (EPOC) blood analysis is used routinely in wildlife veterinary practice to monitor blood oxygenation, but the reliability of the EPOC calculated arterial oxygen-hemoglobin saturation (cSaO 2 ) has never been validated in the white rhinoceros (Ceratotherium simum), despite their susceptibility to hypoxemia during chemical immobilization.
Objectives: We aimed to evaluate the reliability of the EPOC cSaO 2 by comparing it against arterial oxygen-hemoglobin saturation (SaO 2 ) measured by a co-oximeter reference method in immobilized white rhinoceroses.
Methods: Male white rhinoceroses in two studies (both n = 8) were immobilized by darting with different etorphine-based drug combinations, followed by butorphanol or saline (administered intravenously). Animals in both studies received oxygen via intranasal insufflation after 60 min. Blood samples were drawn, at predetermined time points, from a catheter inserted into the auricular artery and analyzed using the EPOC and a co-oximeter. Bland-Altman (to estimate bias and precision) and area root mean squares (ARMS) plots were used to determine the reliability of the EPOC cSaO 2 compared with simultaneous co-oximeter SaO 2 readings.
Results: The rhinoceros were acidotic (pH of 7.3 ± 0.1 [mean ± standard deviation]), hypercapnic (PaCO 2 of 73.7 ± 10.5 mmHg), and normothermic (body temperature of 37.4 ± 1.8°C). In total, 389 paired cSaO 2 -SaO 2 measurements were recorded (the cSaO 2 ranged between 13.2% and 99.0%, and the SaO 2 ranged between 11.8% and 99.9%). The EPOC cSaO 2 readings were unreliable (inaccurate, imprecise, and poor ARMS) across the entire saturation range (bias -6%, precision 5%, and ARMS 8%).
Conclusions: The EPOC cSaO 2 is unreliable and should not be used to monitor blood oxygenation in immobilized white rhinoceroses.
(© 2023 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of the American Society for Veterinary Clinical Pathology.)
Databáze: MEDLINE
Externí odkaz: