Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions.

Autor: Peters JP; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Brahms A; Otto Diels Institute for Organic Chemistry, Kiel University, Otto-Hahn Platz 4, 24098 Kiel, Germany., Janicaud V; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Anikeeva M; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Peschke E; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Ellermann F; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Ferrari A; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Hellmold D; Department of Neurosurgery, University Medical Center Kiel, Arnold-Heller-Str. 3, House D, 24105 Kiel, Germany., Held-Feindt J; Department of Neurosurgery, University Medical Center Kiel, Arnold-Heller-Str. 3, House D, 24105 Kiel, Germany., Kim NM; Institute of Clinical Molecular Biology, Kiel University, Rosalind-Franklin-Straße 12, 24105 Kiel, Germany., Meiser J; Cancer Metabolism Group, Department of Cancer Research, Luxembourg Institute of Health, 6A Rue Nicolas-Ernest Barblé, 1210 Luxembourg, Luxembourg., Aden K; Institute of Clinical Molecular Biology, Kiel University, Rosalind-Franklin-Straße 12, 24105 Kiel, Germany.; Department of Internal Medicine I, University Medical Center Kiel, Kiel, Germany., Herges R; Otto Diels Institute for Organic Chemistry, Kiel University, Otto-Hahn Platz 4, 24098 Kiel, Germany., Hövener JB; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany., Pravdivtsev AN; Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118 Kiel, Germany.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2023 Aug 25; Vol. 9 (34), pp. eadd3643. Date of Electronic Publication: 2023 Aug 23.
DOI: 10.1126/sciadv.add3643
Abstrakt: Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times, enabling in vivo metabolic imaging to be performed noninvasively in real time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1- 15 N-NAM and 1- 15 N nicotinic acid and hyperpolarized them with dDNP, reaching (13.0 ± 1.9)% 15 N polarization. We found that the lifetime of hyperpolarized 1- 15 N-NAM is strongly field- and pH-dependent, with T 1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1- 15 N lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using 15 N dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a 15 N MRI of 1- 15 N-NAM (previously hyperpolarized for more than an hour) in less than 1 s.
Databáze: MEDLINE
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