Controlling the positive ion electrospray ionization of poly(ethylene glycols) when using ultra-high-performance supercritical fluid chromatography-mass spectrometry.

Autor: Cancho-Gonzalez S; Faculty of Engineering & Physical Sciences, School of Chemistry, University of Southampton, Southampton, UK., Ferguson P; New Modalities & Parenteral Development, Pharmaceutical Technology & Development, Operations, AstraZeneca, Macclesfield, UK., Herniman JM; Faculty of Engineering & Physical Sciences, School of Chemistry, University of Southampton, Southampton, UK., Langley GJ; Faculty of Engineering & Physical Sciences, School of Chemistry, University of Southampton, Southampton, UK.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2023 Oct; Vol. 46 (20), pp. e2300425. Date of Electronic Publication: 2023 Aug 23.
DOI: 10.1002/jssc.202300425
Abstrakt: Poly(ethylene glycols) are complex polymers often added to pharmaceutical formulations to improve drug solubility and delivery. One of the main challenges when using chromatographic techniques coupled to mass spectrometry is the unselective ionization of poly(ethylene glycols) oligomers. Additionally, when the chain length is large enough, multiple charged species are formed, further complicating the mass spectra and processing. This study uses the advanced oligomer separation provided by supercritical fluid chromatography with a mass spectrometry approach that selectively ionizes poly(ethylene glycols) as ammoniated molecules to simplify data analysis and facilitate batch-to-batch comparisons. Several visual representations of the response of the ionization events based on the polymer molecular weight and the repeating unit were used to elucidate trends in ionization. Evaluation of the influence of the oligomer length and end-group on the electrospray ionization of the polymer allowed the development of a process to enable selective ionization for these complex polymers.
(© 2023 The Authors. Journal of Separation Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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