Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in.

Autor:	Watanabe Y; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Katsumura E; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Domon T; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Ishikawa Y; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Oguri R; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Takashima M; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Meng Q; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan., Kinoshita M; Graduate School of Agriculture, Kyoto University, Kyoto, Japan., Hashimoto H; Graduate School of Science, Nagoya University, Nagoya, Japan., Hitomi K; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
Jazyk:	angličtina
Zdroj:	Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2023 Oct 25; Vol. 87 (11), pp. 1285-1294.
DOI:	10.1093/bbb/zbad116
Abstrakt:	Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium. (© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
Databáze:	MEDLINE
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