Analysis of CDR1 and MDR1 Gene Expression and ERG11 Substitutions in Clinical Candida tropicalis Isolates from Alexandria, Egypt.

Autor: El-Kholy MA; Department of Microbiology and Biotechnology, Division of Clinical and Biological Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport (AASTMT), Alexandria, Egypt. mohammed.elkholy@aast.edu., Helaly GF; Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt., El Ghazzawi EF; Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt., El-Sawaf G; Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt., Shawky SM; Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Dec; Vol. 54 (4), pp. 2609-2615. Date of Electronic Publication: 2023 Aug 22.
DOI: 10.1007/s42770-023-01106-y
Abstrakt: Introduction: Candida tropicalis is a common non-albicans Candida (NAC) species that causes numerous fungal infections. Increasing antifungal resistance to azoles in NAC is becoming a major health problem worldwide; however, in Egypt, almost no data is available regarding fluconazole resistance mechanisms in C. tropicalis. The current study aims to investigate two possible important molecular mechanisms involved in fluconazole resistance in C. tropicalis isolates.
Materials: Fifty-four clinical C. tropicalis isolates were included. Identification and antifungal susceptibility profiles of the isolates were carried out using the VITEK 2 compact system. The molecular investigation of fluconazole resistance included the expression of the CDR1 and MDR1 genes by quantitative real-time RT-PCR as well as the sequence analysis of the ERG11 gene.
Results: Antifungal susceptibility testing identified 30 fluconazole-non-susceptible isolates. Statistically, CDR1 gene expression in fluconazole-non-susceptible isolates was significantly higher than that in fluconazole-susceptible isolates, with MDR1 gene expression levels that were similar in both non-susceptible and susceptible isolates. Sequence analysis of the ERG11 gene of 26 fluconazole-resistant isolates identified two missense mutations: A395T (Y132F) and G1390A (G464S).
Conclusions: This study has highlighted the role of overexpression of the CDR1 gene and ERG11 gene mutations in fluconazole non-susceptibility. Further studies in Egypt are required to investigate other possible molecular mechanisms involved in azole resistance.
(© 2023. The Author(s).)
Databáze: MEDLINE
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