The effects of 20-hydroxyecdysone on nuclear shape, heterochromatin quantity and gray-level co-occurrence matrix texture analysis of adrenal zona fasciculata cells in an obese gerbil (Gerbillus tarabuli) model for metabolic syndrome: a correlational study.

Autor: Bellahreche Z; LBPO/Nutrition & Metabolism, Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria. zinebbelllahreche@gmail.com., Sihali-Beloui O; LBPO/Nutrition & Metabolism, Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria., Semiane N; LBPO/Nutrition & Metabolism, Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria., Mallek A; LBPO/Nutrition & Metabolism, Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria., Chaouadi M; Pelagic Ecosystem Team, Laboratory of Biological Oceanography and Marine Environment, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria., Fedala A; Faculté de Physique, Laboratoire de Physique des Matériaux, USTHB, Equipe Couches Minces et Semiconducteurs, B.P. 32, El Alia, Bab-ezzouar, 16111, Algiers, Algeria., Dahmani Y; LBPO/Nutrition & Metabolism, Department of Biology and Physiology of Organisms, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.
Jazyk: angličtina
Zdroj: Histochemistry and cell biology [Histochem Cell Biol] 2023 Dec; Vol. 160 (6), pp. 563-576. Date of Electronic Publication: 2023 Aug 22.
DOI: 10.1007/s00418-023-02232-2
Abstrakt: The aim of this study was to reveal the effects of obesity and phytotherapy with 20-hydroxyecdysone (20E) on the nuclei of adrenal zona fasciculata (ZF) in the gerbil Gerbillus tarabuli by analyzing nuclear shape and gray-level co-occurrence matrix (GLCM) texture characteristics and by quantifying heterochromatin. Twelve gerbils were divided into three groups: control (C), HC and HC-20E (animals receiving a high-calorie-diet without or with a supplement of 20E, respectively). The adrenals were removed and fixed for histological and statistical analysis. Principal component analysis showed a positive correlation of area, perimeter and textural correlation in C. Nevertheless, a negative correlation was recorded for contrast and entropy. The obesity caused a disorder in nuclear texture; negative correlation was noted with heterochromatin fraction, which may be related to increased ZF activity. However, administration of 20E seems to improve the nuclear state by preserving circularity, uniformity and homogeneity of nuclei as well as the proportion of heterochromatin, which could be a sign of a downregulation of cell activity.Our results suggest that new techniques of image processing could contribute to the understanding of nuclear changes associated with obesity and its possible therapy in this gerbil model for metabolic syndrome.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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