Induced swimming modified the antioxidant status of gilthead seabream (Sparus aurata).

Autor: Espírito-Santo C; Faculty of Sciences (FCUP), University of Porto, Portugal; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), Portugal. Electronic address: carlos.esanto91@gmail.com., Alburquerque C; Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Spain., Guardiola FA; Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Spain., Ozório ROA; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), Portugal., Magnoni LJ; The New Zealand Institute for Plant and Food Research Limited, Nelson, New Zealand.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2024 Jan; Vol. 269, pp. 110893. Date of Electronic Publication: 2023 Aug 20.
DOI: 10.1016/j.cbpb.2023.110893
Abstrakt: Swimming has relevant physiological changes in farmed fish, although the potential link between swimming and oxidative stress remains poorly studied. We investigated the effects of different medium-term moderate swimming conditions for 6 h on the antioxidant status of gilthead seabream (Sparus aurata), analyzing the activity of enzymes related to oxidative stress in the liver and skeletal red and white muscle. Forty fish were induced to swim individually with the following conditions: steady low (SL, 0.8 body length (BL)·s -1 ), steady high (SH, 2.3 BL·s -1 ), oscillating low (OL, 0.2-0.8 BL·s -1 ) and oscillating high (OH, 0.8-2.3 BL·s -1 ) velocities, and a non-exercised group with minimal water flow (MF, < 0.1 BL·s -1 ). All swimming conditions resulted in lower activities of superoxide dismutase (SOD), glutathione reductase (GR), and glutathione-S-transferase (GST) in the liver compared to the MF group, while steady swimming (SL and SH) led to higher reduced glutathione/oxidized glutathione ratio (GSH/GSSG) compared to the MF condition. Swimming also differently modulated the antioxidant enzyme activities in red and white muscles. The OH condition increased lipid peroxidation (LPO), catalase (CAT) and glutathione peroxidase (GPx) activities in the red muscle, decreasing the GSH/GSSG ratio, whereas the SL condition led to increased GSH. Oscillating swimming conditions (OL and OH) led to lower CAT activity in the white muscle, although GPx activity was increased. The GSH/GSSG ratio in white muscle was increased in all swimming conditions. Liver and skeletal muscle antioxidant status was modulated by exercise, highlighting the importance of adequate swimming conditions to minimize oxidative stress in gilthead seabream.
Competing Interests: Declaration of Competing Interest The authors declare no competing financial interests.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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