Biomimetic behaviors in hydrogel artificial cells through embedded organelles.

Autor: Allen ME; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Hindley JW; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., O'Toole N; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Cooke HS; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Contini C; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Law RV; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Ces O; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK., Elani Y; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub, London W12 0BZ, UK.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Aug 29; Vol. 120 (35), pp. e2307772120. Date of Electronic Publication: 2023 Aug 21.
DOI: 10.1073/pnas.2307772120
Abstrakt: Artificial cells are biomimetic structures formed from molecular building blocks that replicate biological processes, behaviors, and architectures. Of these building blocks, hydrogels have emerged as ideal, yet underutilized candidates to provide a gel-like chassis in which to incorporate both biological and nonbiological componentry which enables the replication of cellular functionality. Here, we demonstrate a microfluidic strategy to assemble biocompatible cell-sized hydrogel-based artificial cells with a variety of different embedded functional subcompartments, which act as engineered synthetic organelles. The organelles enable the recreation of increasingly biomimetic behaviors, including stimulus-induced motility, content release through activation of membrane-associated proteins, and enzymatic communication with surrounding bioinspired compartments. In this way, we showcase a foundational strategy for the bottom-up construction of hydrogel-based artificial cell microsystems which replicate fundamental cellular behaviors, paving the way for the construction of next-generation biotechnological devices.
Databáze: MEDLINE
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