Effect of different levels of EDTA on phytoextraction of heavy metal and growth of Brassica juncea L.

Autor: Kamal MA; Department of Civil Engineering, College of Engineering, King Saud University, Riyadh, Saudi Arabia., Perveen K; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia., Khan F; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia., Sayyed RZ; Faculty of Health and Life Sciences, INTI International University, Nilai, Negeri Sembilan, Malaysia., Hock OG; Faculty of Health and Life Sciences, INTI International University, Nilai, Negeri Sembilan, Malaysia., Bhatt SC; Faculty of Agriculture, Kumaun University, Nainital, Uttarakhand, India., Singh J; Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, India., Qamar MO; Department of Civil Engineering (Environmental Science and Engineering), Yeungnam University, Gyeongsan, Republic of Korea.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2023 Aug 03; Vol. 14, pp. 1228117. Date of Electronic Publication: 2023 Aug 03 (Print Publication: 2023).
DOI: 10.3389/fmicb.2023.1228117
Abstrakt: Heavy metal pollution of soil is a major concern due to its non-biodegradable nature, bioaccumulation, and persistence in the environment. To explore the probable function of EDTA in ameliorating heavy metal toxicity and achieve the sustainable development goal (SDG), Brassica juncea L. seedlings were treated with different concentrations of EDTA (0, 1.0, 2.0, 3.0, and 4.0 mM Kg -1 ) in heavy metal-polluted soil. Plant samples were collected 60 days after sowing; photosynthetic pigments, H 2 O 2 , monoaldehyde (MDA), antioxidant enzymes, and ascorbic acid content, as well as plant biomass, were estimated in plants. Soil and plant samples were also examined for the concentrations of Cd, Cr, Pb, and Hg. Moreover, values of the phytoremediation factor were utilized to assess the accumulation capacity of heavy metals by B. juncea under EDTA treatments. In the absence of EDTA, B. juncea seedlings accrued heavy metals in their roots and shoots in a concentration-dependent manner. However, the highest biomass of plants (roots and shoots) was recorded with the application of 2 mM kg -1 EDTA. Moreover, high levels (above 3 mM kg -1 ) of EDTA concentration have reduced the biomass of plants (roots and shoots), photosynthetic area, and chlorophyll content. The effect of EDTA levels on photosynthetic pigments (chlorophyll a and b) revealed that with an increment in EDTA concentration, accumulation of heavy metals was also increased in the plant, subsequently decreasing the chlorophyll a and b concentration in the plant. TLF was found to be in the order Pb> Hg> Zn> and >Ni, while TF was found to be in the order Hg>Zn>Ni>Pb, and the best dose was 3 mM kg -1 EDTA for Hg and 4 mM kg -1 for Pb, Ni, and Zn. Furthermore, hyperaccumulation of heavy metals enhanced the generation of hydrogen peroxide (H 2 O 2 ), superoxide anions (O 2 •- ), and lipid peroxidation. It also interrupts mechanisms of the antioxidant defense system. Furthermore, heavy metal stress reduced plant growth, biomass, and chlorophyll (chl) content. These findings suggest that the exogenous addition of EDTA to the heavy metal-treated seedlings increases the bioavailability of heavy metals for phytoextraction and decreases heavy metal-induced oxidative injuries by restricting heavy metal uptake and components of their antioxidant defense systems.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Kamal, Perveen, Khan, Sayyed, Hock, Bhatt, Singh and Qamar.)
Databáze: MEDLINE
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