Controlling vegetative cells and spores growth of Bacillus spp. using ethanolic Ketapang ( Terminalia catappa L.) leaf extract.

Autor:	Madhavan K; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia., Rukayadi Y; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia.; Natural Medicines and Products Research Laboratory (NaturMeds), Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia., Abdul-Mutalib NA; Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia.; Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Jul 27; Vol. 9 (8), pp. e18749. Date of Electronic Publication: 2023 Jul 27 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e18749
Abstrakt:	Terminalia catappa L. is a large, spreading type of tree which usually grows in tropical environment, especially at coastal area with sandy stones. The current study evaluated anti- Bacillus potential of the ethanolic ketapang ( Terminalia catappa L.) leaf extract (EKLE) as antibacterial and sporicidal agent against vegetative cells and spores of Bacillus spp. The antibacterial activity of EKLE against Bacillus spp. ( B . cereus ATCC33019, B . pumilus ATCC14884, B . subtilis ATCC6633 and B . megaterium ATCC14581) vegetative cells were determined by performing well diffusion assay (WDA), minimum inhibition concentration (MIC), minimum bacterial concentration (MBC) and time-kill curve analyses. The sporicidal activity was tested at different concentrations of EKLE. Then, the extract's stability in terms of antibacterial and sporicidal activities upon exposure to different temperatures and pHs were carried out. Results demonstrated inhibition zones of EKLE against Bacillus spp. was in the range of 9.25 ± 0.75 mm - 11.67 ± 0.47 mm. All vegetative cells of Bacillus spp. were inhibited with MIC values at 0.63-1.25 mg/mL and can be completely killed with MBC values of 0.63 - >5.00 mg/mL. Time-kill analysis showed all the Bacillus spp. tested can be completely killed at concentrations of 2.50-5.00 mg/mL from 1 to 4 h. EKLE concentration of 1% (w/v) completely killed all Bacillus spp. spores at different exposure time. The antibacterial and sporicidal activities of EKLE were not affected by exposure to different temperatures (4, 30, 50, 80 and 121 °C) and pHs (3, 7 and 10), revealing the stability of the extract against different conditions. In conclusion, Terminalia catappa L. leaf exhibits antibacterial and sporicidal activities against Bacillus spp., therefore, the extract can be developed as anti- Bacillus agent, paving the way for its utilization in food industry as a natural food preservative. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 Published by Elsevier Ltd.)
Databáze:	MEDLINE
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