Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study.

Autor:	Stocco E; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; Department of Cardiac, Thoracic and Vascular Science and Public Health, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, Italy.; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Barbon S; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Faccio D; Plastic and Reconstructive Surgery Unit, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Petrelli L; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy., Incendi D; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy., Zamuner A; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy.; Department of Civil, Environmental and Architectural Engineering University of Padova, Via Francesco Marzolo 9, 35131, Padova, Italy.; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, Italy., De Rose E; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy., Confalonieri M; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, Italy., Tolomei F; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, Italy., Todros S; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, Italy., Tiengo C; Plastic and Reconstructive Surgery Unit, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Macchi V; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Dettin M; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy.; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, Italy., De Caro R; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy., Porzionato A; Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy.
Jazyk:	angličtina
Zdroj:	Materials today. Bio [Mater Today Bio] 2023 Aug 05; Vol. 22, pp. 100761. Date of Electronic Publication: 2023 Aug 05 (Print Publication: 2023).
DOI:	10.1016/j.mtbio.2023.100761
Abstrakt:	In severe peripheral nerve injuries, nerve conduits (NCs) are good alternatives to autografts/allografts; however, the results the available devices guarantee for are still not fully satisfactory. Herein, differently bioactivated NCs based on the new polymer oxidized polyvinyl alcohol (OxPVA) are compared in a rat model of sciatic nerve neurotmesis (gap: 5 mm; end point: 6 weeks). Thirty Sprague Dawley rats are randomized to 6 groups: Reverse Autograft (RA); Reaxon®; OxPVA; OxPVA + EAK (self-assembling peptide, mechanical incorporation); OxPVA + EAK-YIGSR (mechanical incorporation); OxPVA + Nerve Growth Factor (NGF) (adsorption). Preliminarily, all OxPVA-based devices are comparable with Reaxon® in Sciatic Functional Index score and gait analysis; moreover, all conduits sustain nerve regeneration (S100, β-tubulin) without showing substantial inflammation (CD3, F4/80) evidences. Following morphometric analyses, OxPVA confirms its potential in PNI repair (comparable with Reaxon®) whereas OxPVA + EAK-YIGSR stands out for its myelinated axons total number and density, revealing promising in injury recovery and for future application in clinical practice. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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