Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.

Autor:	Lantos E; Julius Kühn-Institut, Federal Research Centre of Cultivated Plants, Institute of Breeding Research on Horticultural Crops, Quedlinburg, Germany., Krämer R; Julius Kühn-Institut, Federal Research Centre of Cultivated Plants, Institute of Breeding Research on Horticultural Crops, Quedlinburg, Germany., Richert-Pöggeler KR; Julius Kühn-Institut, Federal Research Centre of Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany., Maiss E; Leibniz-University Hannover, Institute of Horticultural Production Systems, Hannover, Germany., König J; Julius Kühn-Institut, Federal Research Centre of Cultivated Plants, Institute of Breeding Research on Horticultural Crops, Quedlinburg, Germany., Nothnagel T; Julius Kühn-Institut, Federal Research Centre of Cultivated Plants, Institute of Breeding Research on Horticultural Crops, Quedlinburg, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Jul 31; Vol. 14, pp. 1187563. Date of Electronic Publication: 2023 Jul 31 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1187563
Abstrakt:	Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest. (Copyright © 2023 Lantos, Krämer, Richert-Pöggeler, Maiss, König and Nothnagel.)
Databáze:	MEDLINE
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