Human Hepatocellular response in Cholestatic Liver Diseases.

Autor: Ortiz K; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Cetin Z; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Sun Y; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Hu Z; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Kurihara T; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Tafaleng EN; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Florentino RM; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.; Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Ostrowska A; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.; Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Soto-Gutierrez A; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.; Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; University of Pittsburgh Drug Discovery Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, USA., Faccioli LAP; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.; Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Jazyk: angličtina
Zdroj: Organogenesis [Organogenesis] 2023 Dec 31; Vol. 19 (1), pp. 2247576.
DOI: 10.1080/15476278.2023.2247576
Abstrakt: Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), the most common types of cholestatic liver disease (CLD), result in enterohepatic obstruction, bile acid accumulation, and hepatotoxicity. The mechanisms by which hepatocytes respond to and cope with CLD remain largely unexplored. This study includes the characterization of hepatocytes isolated from explanted livers of patients with PBC and PSC. We examined the expression of hepatocyte-specific genes, intracellular bile acid (BA) levels, and oxidative stress in primary-human-hepatocytes (PHHs) isolated from explanted livers of patients with PBC and PSC and compared them with control normal human hepatocytes. Our findings provide valuable initial insights into the hepatocellular response to cholestasis in CLD and help support the use of PHHs as an experimental tool for these diseases.
Databáze: MEDLINE