Evolution Toward Chip-Based Arrays in the Laboratory Diagnosis of Human Allergic Disease.
| Autor: | Hamilton RG; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Md. Electronic address: rhamilt2@jh.edu., Croote D; IgGenix, Inc, South San Francisco, Calif., Lupinek C; Macro Array Diagnostics, Vienna, Austria., Matsson P; Clinical Laboratory Standards Institute, Malvern, Pa. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | The journal of allergy and clinical immunology. In practice [J Allergy Clin Immunol Pract] 2023 Oct; Vol. 11 (10), pp. 2991-2999. Date of Electronic Publication: 2023 Aug 18. |
| DOI: | 10.1016/j.jaip.2023.08.017 |
| Abstrakt: | Multiplex-based specific IgE antibody assays have emerged into the clinical immunology laboratory through the combined use of pure, recombinant allergenic molecules and new methods to simultaneously and accurately analyze specific IgE antibodies to hundreds of allergens. This review traces the historical development and examines outstanding questions related to the strengths and limitations of these new molecular allergen multipex technologies for the assessment of human allergic sensitization. Multiplexed technologies are poised to provide the most cost-effective and comprehensive evaluation of patients with suspected allergy as compared with the commonly used singleplex autoanalyzers. How analytically sensitive and quantitative are the multiplex technologies, down to 0.1 kU (Copyright © 2023 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|