Kaempferol induces programmed cell death in Naegleria fowleri.
Autor: | Lê HG; Department of Parasitology and Tropical Medicine, Institute of Health Science, Gyeongsang National University College of Medicine, Jinju 52727, Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea., Kang JM; Department of Parasitology and Tropical Medicine, Institute of Health Science, Gyeongsang National University College of Medicine, Jinju 52727, Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea., Võ TC; Department of Parasitology and Tropical Medicine, Institute of Health Science, Gyeongsang National University College of Medicine, Jinju 52727, Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea., Na BK; Department of Parasitology and Tropical Medicine, Institute of Health Science, Gyeongsang National University College of Medicine, Jinju 52727, Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea. Electronic address: bkna@gnu.ac.kr. |
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Jazyk: | angličtina |
Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2023 Oct; Vol. 119, pp. 154994. Date of Electronic Publication: 2023 Jul 23. |
DOI: | 10.1016/j.phymed.2023.154994 |
Abstrakt: | Background: Naegleria fowleri is a brain-eating amoeba causing a fatal brain infection called primary amoebic meningoencephalitis (PAM). Despite its high mortality over 95%, effective therapeutic drug for PAM has not been developed yet. Therefore, development of an effective and safe therapeutic drug for PAM is urgently needed. In this study, we investigated anti-amoebic effect of kaempferol (KPF) against N. fowleri and its underlying anti-amoebic molecular mechanisms. Methods: Anti-amoebic activity of KPF against N. fowleri trophozoites, as well as cytotoxicity of KPF in C6 glial cells and CHO-K1 cells were investigated. The programmed cell death mechanisms in KPF-treated N. fowleri were also analyzed by apoptosis-necrosis assay, mitochondrial dysfunction assay, TUNEL assay, RT-qPCR, and CYTO-ID assay. Results: KPF showed anti-amoebic activity against N. fowleri trophozoites with an IC Conclusion: KPF induces programmed cell death in N. fowleri trophozoites via apoptosis-like pathway and autophagy pathway. KPF could be used as a candidate of anti-amoebic drug or supplement compound in the process of developing or optimizing therapeutic drug for PAM. Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest. (Copyright © 2023 Elsevier GmbH. All rights reserved.) |
Databáze: | MEDLINE |
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