Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes.

Autor: Chylinski C; Animal and Veterinary Sciences, Scotland's Rural College, Easter Bush, Roslin, EH25 9RG, UK., Degnes KF; Department of Biotechnology and Nanomedicine, SINTEF, 7465, Trondheim, Norway., Aasen IM; Department of Biotechnology and Nanomedicine, SINTEF, 7465, Trondheim, Norway., Ptochos S; Department of Animal Health, Animal Welfare and Food Safety, Norwegian Veterinary Institute, 1433, Ås, Norway., Blomstrand BM; Norwegian Centre for Organic Agriculture, Gunnarsveg 6, 6630, Tingvoll, Norway., Mahnert KC; Norwegian Institute of Wood Technology, 0373, Oslo, Norway., Enemark HL; Department of Animal Health, Animal Welfare and Food Safety, Norwegian Veterinary Institute, 1433, Ås, Norway., Thamsborg SM; Veterinary Parasitology, University of Copenhagen, Dyrlægevej 100, 1870, Frederiksberg, Denmark., Steinshamn H; Division of Food Production and Society, Grasslands and Livestock, Norwegian Institute of Bioeconomy Research, 6630, Tingvoll, Norway., Athanasiadou S; Animal and Veterinary Sciences, Scotland's Rural College, Easter Bush, Roslin, EH25 9RG, UK. spiridoula.athanasiadou@sruc.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Aug 18; Vol. 13 (1), pp. 13498. Date of Electronic Publication: 2023 Aug 18.
DOI: 10.1038/s41598-023-38476-0
Abstrakt: The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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