Nano-residronate loaded κ-carrageenan-based injectable hydrogels for bone tissue regeneration.

Autor: Haroon B; Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan., Sohail M; Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan; Faculty of Pharmacy, Cyprus International University, Nicosia 99258, North Cyprus. Electronic address: msmarwat@gmail.com., Minhas MU; College of Pharmacy, University of Sargodha, Sargodha 40100, Punjab, Pakistan., Mahmood A; Collage of Pharmacy, Al Ain University, Abu Dhabi, United Arab Emirates; AAU Health and Biomedical Research Center, Al Ain University, Abu Dhabi, United Arab Emirates., Hussain Z; Department of Pharmaceutics & Pharmaceutical Technology, College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates., Ahmed Shah S; Department of Biosystems and Soft Matters, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw 02-106, Poland; Faculty of Pharmacy, Superior University, Lahore, Pakistan., Khan S; Center of Pharmaceutical Engineering Science (CPES), School of Pharmacy and Biomedical Science, University of Bradford, BD7,1DP, United Kingdom., Abbasi M; Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan., Kashif MUR; Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Nov 01; Vol. 251, pp. 126380. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1016/j.ijbiomac.2023.126380
Abstrakt: Bone tissue possesses intrinsic regenerative capabilities to address deformities; however, its ability to repair defects caused by severe fractures, tumor resections, osteoporosis, joint arthroplasties, and surgical reconsiderations can be hindered. To address this limitation, bone tissue engineering has emerged as a promising approach for bone repair and regeneration, particularly for large-scale bone defects. In this study, an injectable hydrogel based on kappa-carrageenan-co-N-isopropyl acrylamide (κC-co-NIPAAM) was synthesized using free radical polymerization and the antisolvent evaporation technique. The κC-co-NIPAAM hydrogel's cross-linked structure was confirmed using Fourier transform infrared spectra (FTIR) and nuclear magnetic resonance ( 1 H NMR). The hydrogel's thermal stability and morphological behavior were assessed using thermogravimetric analysis (TGA) and scanning electron microscopy (SEM), respectively. Swelling and in vitro drug release studies were conducted at varying pH and temperatures, with minimal swelling and release observed at low pH (1.2) and 25 °C, while maximum swelling and release occurred at pH 7.4 and 37oC. Cytocompatibility analysis revealed that the κC-co-NIPAAM hydrogels were biocompatible, and hematoxylin and eosin (H&E) staining demonstrated their potential for tissue regeneration and enhanced bone repair compared to other experimental groups. Notably, digital x-ray examination using an in vivo bone defect model showed that the κC-co-NIPAAM hydrogel significantly improved bone regeneration, making it a promising candidate for bone defects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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