Electron coupling effect-triggered monatomic copper laccase-mimicking nanozyme for the degradation and detection of guaiacol produced by Alicyclobacillus acidoterrestris.

Autor: Niu X; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Wu L; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Wu F; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Guan J; Institute of Physical Chemistry, College of Chemistry, Jilin University, 2519 Jiefang Road, Changchun, 130021, PR China. Electronic address: guanjq@jlu.edu.cn., Wang H; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China. Electronic address: wanghs@jlu.edu.cn.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2023 Oct 15; Vol. 238, pp. 115606. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1016/j.bios.2023.115606
Abstrakt: The generation of guaiacol by Alicyclobacillus acidoterrestris (A. acidoterrestris) in fruit juices negatively affects public health and causes severe environmental pollution. Therefore, the sensitive detection and efficient degradation of guaiacol in real samples are crucial. Here, we develop an electrochemical sensor utilizing a copper single-atom nanozyme (CuN 4 -G) to detect and degrade guaiacol at the picomolar level. Density functional theory (DFT) calculations verify that the bonding electron coupling effect in the CuN 4 -G facilitates rapid electron transfer, enhances electrical conductivity, and provides abundant active sites, thereby leading to exceptional catalytic performance. Moreover, CuN 4 -G demonstrates a K m value similar to that of natural laccase but a higher V max , highlighting its potential as a highly efficient biocatalyst. The CuN 4 -G-based electrochemical sensor achieves a detection from 5 to 50,000 pM for guaiacol, with a 1.2 pM (S/N = 3) detection limit. Additionally, CuN 4 -G-modified electrodes display high selectivity and excellent stability. CuN 4 -G nanozyme can keep its activity in conditions of pH (3-9), temperature (30-90 °C), ionic strength (0-400 mM), and organic solvent (0-50% (v/v)), overcoming the deficiencies of natural enzymes. Furthermore, our electrochemical sensor can not only accurately detect guaiacol, but also degrade it in actual fruit juice samples infected by A. acidoterrestris, demonstrating its potential applications in food and environmental monitoring.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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