Triple contrast computed tomography reveals site-specific biomechanical differences in the human knee joint-A proof of concept study.

Autor: Orava H; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland., Paakkari P; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland., Jäntti J; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland., Honkanen MKM; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland., Honkanen JTJ; Center of Oncology, Kuopio University Hospital, Kuopio, Finland., Virén T; Center of Oncology, Kuopio University Hospital, Kuopio, Finland., Joenathan A; Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, Boston, Massachusetts, USA., Tanska P; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland., Korhonen RK; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland., Grinstaff MW; Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, Boston, Massachusetts, USA., Töyräs J; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Science Service Center, Kuopio University Hospital, Kuopio, Finland.; School of Electrical Engineering and Computer Science, The University of Queensland, Brisbane, Australia., Mäkelä JTA; Department of Technical Physics, University of Eastern Finland, Kuopio, Finland.; Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2024 Feb; Vol. 42 (2), pp. 415-424. Date of Electronic Publication: 2023 Aug 30.
DOI: 10.1002/jor.25683
Abstrakt: Cartilage and synovial fluid are challenging to observe separately in native computed tomography (CT). We report the use of triple contrast agent (bismuth nanoparticles [BiNPs], CA4+, and gadoteridol) to image and segment cartilage in cadaveric knee joints with a clinical CT scanner. We hypothesize that BiNPs will remain in synovial fluid while the CA4+ and gadoteridol will diffuse into cartilage, allowing (1) segmentation of cartilage, and (2) evaluation of cartilage biomechanical properties based on contrast agent concentrations. To investigate these hypotheses, triple contrast agent was injected into both knee joints of a cadaver (N = 1), imaged with a clinical CT at multiple timepoints during the contrast agent diffusion. Knee joints were extracted, imaged with micro-CT (µCT), and biomechanical properties of the cartilage surface were determined by stress-relaxation mapping. Cartilage was segmented and contrast agent concentrations (CA4+ and gadoteridol) were compared with the biomechanical properties at multiple locations (n = 185). Spearman's correlation between cartilage thickness from clinical CT and reference µCT images verifies successful and reliable segmentation. CA4+ concentration is significantly higher in femoral than in tibial cartilage at 60 min and further timepoints, which corresponds to the higher Young's modulus observed in femoral cartilage. In this pilot study, we show that (1) large BiNPs do not diffuse into cartilage, facilitating straightforward segmentation of human knee joint cartilage in a clinical setting, and (2) CA4+ concentration in cartilage reflects the biomechanical differences between femoral and tibial cartilage. Thus, the triple contrast agent CT shows potential in cartilage morphology and condition estimation in clinical CT.
(© 2023 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.)
Databáze: MEDLINE
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