| Autor: |
Wilson WE, Harvan DJ, Hamm C, Lazarus LH, Klapper DG, Yajima H, Hayashi Y |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of peptide and protein research [Int J Pept Protein Res] 1986 Jul; Vol. 28 (1), pp. 58-66. |
| DOI: |
10.1111/j.1399-3011.1986.tb03229.x |
| Abstrakt: |
Two physalaemin (PHY)-like immunoreactive peptides, designated PHLIPs, have been purified from extracts of rabbit stomach tissue. Fast atom bombardment/mass spectrometry (FAB/MS) indicated that the m/z values for the PHLIP protonated molecular ions were 867.419 and 796.4. FAB/tandem MS spectra, coupled with a knowledge of the amino acid composition and the aid of a computerized fragment-matching program, indicated the amino acid sequences to be: (formula; see text) The sequences of PHLIPs-7 and -8 were confirmed with synthetic peptides. The PHY-antiserum cross-reactivity of the PHLIPs reflects homology at amino acid residues 1, 3, 4 and 5 for the mammalian and amphibian residues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Plný text