Sheep as a Model for Liver Transplantation.

Autor:	Alsebayel M; General Surgery, Alhabib Medical Group, Riyadh, SAU., El-Sheikh YM; Liver Transplantation and Hepatobiliary-Pancreatic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, SAU., Al-Mohanna FH; Comparative Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, SAU., Al-Abbad SI; Liver Transplantation and Hepatobiliary-Pancreatic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, SAU., Al-Jammali A; Perfusion Services, King Faisal Specialist Hospital and Research Centre, Riyadh, SAU., Alsebayel YM; General Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, SAU., Al-Bahli HM; Organ Transplant Center, Prince Sultan Military Medical City, Riyadh, SAU.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2023 Jul 17; Vol. 15 (7), pp. e42002. Date of Electronic Publication: 2023 Jul 17 (Print Publication: 2023).
DOI:	10.7759/cureus.42002
Abstrakt:	Objective: Experimental animal liver transplantation is the initial step, before the application of the procedure on humans. Canine and swine transplantation were used to perfect the technical aspects of the procedure. Small animals such as rats were mainly utilized to study the metabolic and immunological aspects of liver transplantation. In this paper, we describe our experience with attempting liver transplantation in a sheep animal model. Material and Method: The animal model used for both donor and recipient was outbred male weanling sheep of Naimi strain ( Ovis aries, Awassi). They weigh between 25 and 35 kg. They were put under general anesthesia. Harvested livers were kept in cold storage. Recipients underwent hepatectomy, after construction of an active portal systemic bypass using a Medtronic pump. The implantation was done with caval replacement and direct portal anastomosis. The hepatic artery with its attachments to the aortal was anastomosed directly to the recipient aorta. Result: Twelve pairs (24 sheep) were utilized for donor and recipient surgery. Donor surgery was completed successfully in all 12 cases. Recipient surgery was not completed in three cases, when animals were lost in the implantation phase, before reperfusion mainly due to uncontrolled bleeding, resulting in hemodynamic instability. We also lost five recipients immediately after reperfusion, mainly due to post-perfusion bleeding and hemodynamic instability. Four recipients stayed alive after the implantation. Conclusion: We demonstrated the feasibility of using sheep as an animal model for liver transplantation. We described the similarities of sheep liver to that of humans, as well as the technical difficulties. This model is suitable in situations where other well-established models are not available for cultural or religious reasons. Further refinement in the technical aspects will be needed, as well as investigation of the biochemical outcome and long-term survival. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2023, Alsebayel et al.)
Databáze:	MEDLINE
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