Paraburkholderia flagellata sp. nov. and Paraburkholderia adhaesiva sp. nov., two novel species isolated from forest soil in Dinghushan Biosphere Reserve in Guangdong, China.

Autor: Guo XY; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China., Wu KC; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China., Dong CZ; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China., Zhang QM; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China., Qiu LH; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. qiulh@mail.sysu.edu.cn.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2023 Oct; Vol. 116 (10), pp. 1023-1035. Date of Electronic Publication: 2023 Aug 17.
DOI: 10.1007/s10482-023-01867-4
Abstrakt: Two Gram-stain-negative, aerobic, motile and short rod strains, designated 4D117 T and ZD32-2 T , were isolated from the forest soils. Strains 4D117 T and ZD32-2 T grew optimally at pH 4.0-6.5, 20-33 °C and pH 4.5-7.0, 33 °C, respectively, and both at 0.5% (w/v) NaCl concentration. Strains 4D117 T and ZD32-2 T shared the highest 16S rRNA gene sequence similarity with P. acidiphila 7Q-K02 T  (99.1%) and P. ferrariae NBRC 106233 T (98.7%), respectively. The genome size and G + C contents of strains 4D117 T and ZD32-2 T were 9,002,095 bp, 62.9% and 6,974,420 bp, 61.7%, respectively. The dDDH and ANI values between strains 4D117 T , ZD32-2 T and closely related Paraburkholderia species were in the ranges of 21.9-51.6% and 82.9-94.4%, and 81.7% and 25.4% between themself, respectively. Functional genomic analysis showed both strains were capable of degrading contaminants, such as benzoate, anthranilic acid and catechol for 4D117 T , and benzene and catechol for ZD32-2 T , indicating that they may have potentials for soil pollutant treatment. The main polar lipids of strains 4D117 T and ZD32-2 T were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain 4D117 T contained C 16:0 , C 19:0 cyclo ω8c and C 18:1 ω7c and/or C 18:1 ω6c, while strain ZD32-2 T had C 16:0 and C 17:0 cyclo as their major cellular fatty acids (> 10%). Based on the phenotypic characters and genomic data, strains 4D117 T and ZD32-2 T represent two novel species of genus Paraburkholderia, for which the names Paraburkholderia flagellata sp. nov. (type strain 4D117 T  = GDMCC 1.2617 T  = NBRC 115278 T ) and Paraburkholderia adhaesiva sp. nov. (type strain ZD32-2 T  = GDMCC 1.2622 T  = NBRC 115282 T ) are proposed.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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