Evaluation of the relationship between de-novo DSA development and CXCR5+PD-1+CD8+ T cells and PD-1/PD-L1 mRNA expression in kidney transplant recipients.

Autor: Ekinci NS; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, Turkey., Yilmaz VT; Division of Nephrology, Department of Internal Medicine, Akdeniz University Faculty of Medicine, Antalya, Turkey.; Prof. Dr. Tuncer Karpuzoglu Transplantation Center, Akdeniz University Hospital, Antalya, Turkey., Kilinc Y; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, Turkey., Kocak H; Division of Nephrology, Department of Internal Medicine, Akdeniz University Faculty of Medicine, Antalya, Turkey.; Prof. Dr. Tuncer Karpuzoglu Transplantation Center, Akdeniz University Hospital, Antalya, Turkey., Darbas S; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, Turkey., Kisaoglu A; Prof. Dr. Tuncer Karpuzoglu Transplantation Center, Akdeniz University Hospital, Antalya, Turkey.; Department of General Surgery, Akdeniz University Faculty of Medicine, Antalya, Turkey., Aydinli B; Prof. Dr. Tuncer Karpuzoglu Transplantation Center, Akdeniz University Hospital, Antalya, Turkey.; Department of General Surgery, Akdeniz University Faculty of Medicine, Antalya, Turkey., Uzuner SY; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, Turkey., Ucar F; Department of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, Turkey.; Prof. Dr. Tuncer Karpuzoglu Transplantation Center, Akdeniz University Hospital, Antalya, Turkey.
Jazyk: angličtina
Zdroj: Clinical transplantation [Clin Transplant] 2023 Nov; Vol. 37 (11), pp. e15104. Date of Electronic Publication: 2023 Aug 17.
DOI: 10.1111/ctr.15104
Abstrakt: Background and Aims: The relationship between the Follicular Cytotoxic T cell subgroup and expression levels of PD1/PD-L1 genes and the development of donor specific antibody (DSA) is unknown. In this study, we aimed to examine CD8+CXCR5+PD-1+ follicular cytotoxic T cell levels and expression levels of PD1/PD-L1 genes in peripheral blood lymphocytes in de-novo DSA positive and negative kidney transplant recipients (KTR).
Methods: In our study, expression of PD-1/ PD-L1 genes by Real-Time Quantitative PCR method and CD8+CXCR5+PD-1+ T cell expression levels by flow cytometric method were obtained from peripheral blood samples. 63 participants were included in the study (de-novo DSA positive recipients (n = 22, group 1), de-novo DSA negative recipients (n = 20, group 2) and healthy control (n = 21, group 3). All patients had negative PRA before kidney transplantation. Expression (%) levels of target cells were evaluated by flow cytometry method. IBM SPSS Statistics for Windows Version 22 and R.3.3.2 software were used to evaluate the data.
Results: The demographic data of the groups were similar. PD-1 mRNA expression was higher in de-novo DSA positive KTR than negative (respectively, 1.03 ± .29/.82 ± .15, p: .001). CD8+CXCR5+PD-1+ T cell expression levels were found to be higher in the de-novo DSA positive group than in the negative group and similar to the healthy group (respectively, 3.06 ± 1.98/.52 ± .40, p:.001, 3.06 ± 1.98/2.78 ± .59, p:.62). The percentage of CD8+CXCR5+PD-1+ expressing T cells was significantly lower in the HLA-Class II+ group than other groups (HLA CI/II/ I+II, respectively, 3.63 ± 2.72/1.65 ± .50/3.68 ± 1.67, p: .04).
Conclusions: In our study, a significant relationship was found between DSA formation and PD-1 mRNA level and CD8+CXCR5+PD-1+ follicular cytotoxic T cell in KTR.
(© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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