Printed Divisional Optical Biochip for Multiplex Visualizable Exosome Analysis at Point-of-Care.

Autor: Yang X; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Zhang Z; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Wu Y; Department of Emergency, the Seventh Medical Center, Chinese PLA General Hospital, Beijing, 100700, P. R. China., Wang H; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Yun Y; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Sun Y; School of Physics and Engineering, ITMO University, Saint Petersburg, 197101, Russia., Xie H; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Bogdanov B; School of Physics and Engineering, ITMO University, Saint Petersburg, 197101, Russia., Senyushkin P; School of Physics and Engineering, ITMO University, Saint Petersburg, 197101, Russia., Chi J; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Lian Z; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Wu D; Department of Neurosurgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, 100853, P. R. China., Su M; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China., Song Y; Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.; University of Chinese Academy of Sciences (UCAS), Beijing, 100049, P. R. China.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Jan; Vol. 36 (4), pp. e2304935. Date of Electronic Publication: 2023 Dec 03.
DOI: 10.1002/adma.202304935
Abstrakt: Rapid detection of various exosomes is of great significance in early diagnosis and postoperative monitoring of cancers. Here, a divisional optical biochip is reported for multiplex exosome analysis via combining the self-assembly of nanochains and precise surface patterning. Arising from resonance-induced near-field enhancement, the nanochains show distinct color changes after capturing target exosomes for direct visual detection. Then, a series of divisional nanochain-based biochips conjugated with several specific antibodies are fabricated through designed hydrophilic and hydrophobic patterns. Because of the significant wettability difference, one sample droplet is precisely self-splitting into several microdroplets enabling simultaneous identification of multiple target exosomes in 30 min with a sensitivity of 6 × 10 7 particles mL -1 , which is about two orders lower than enzyme-linked immunosorbent assay. Apart from the trace amount detection, excellent semiquantitative capability is demonstrated to distinguish clinical exosomes from glioblastoma patients and healthy people. This method is simple, versatile, and highly efficient that can be extended as a diagnostic tool for many diseases, promoting the development of liquid biopsy.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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