| Autor: |
Munir S; Sarhad Institute of Allied Health Sciences, Sarhad University of Science & Technology, Peshawar, Pakistan., Amanat T; Allama Iqbal Medical College, Lahore, Pakistan., Arshad Raja M; Fatima Jinnah Medical University Lahore, Pakistan., Mohammed K; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia., Ahmed Rasheed R; Histology & Cell Biology Department, Faculty of Medicine, King Salman International University, South Sinai, Egypt., S Hussein D; Department of Chemistry, College of Sciences and Health, Cleveland State University, Cleveland, USA., Shireen F; Sarhad Institute of Allied Health Sciences, Sarhad University of Science & Technology, Peshawar, Pakistan., Ahmad J; Department of Microbiology, Immunology, Infectious Diseases, Transplantation and Related Diseases, University of Rome, Tor Vergata, Italy., Ahmad Z; Department of Microbiology, Hazara University, Mansehra, Pakistan., Hayat S; Department of Microbiology, Hazara University, Mansehra, Pakistan. |
| Abstrakt: |
The current research investigation demonstrated that the aqueous leaves extract of Rosamarinus officinalis possesses cardinal phyto-chemicals to fabricate AgNPs in an eco-friendly way. The phyto-synthesized AgNPs were characterized to be stable, monodispersed, polycrystalline and mostly spheroidal in conformation. The nano-spheriods were observed to be 25-75 nm in diameter, displaying λ max peak at 430 nm. From the comparative antimicrobial investigations, it was observed that AgNPs manifested tremendous bactericidal properties against all test organisms particularly S. epidermis (89%), S. aureus (84%) and K. pneumonia (84%), owing least MIC values of 40µL. The aced fungicidal activity was also exhibited by AgNPs against all fungal test species particularly C. herbarum (90%), A. flavus (85%), R. stolonifer (85%) and C. jadinii (85%). In contrast to AgNPs, all crude ethanolic, aqueous, methanolic and n-hexanoic extracts manifested less to moderate antimicrobial activity against all test micro-organisms with three-fold escalating MIC values i.e., 160µL. |
