Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes.

Autor: Costa BF; Laboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas Institute, Belém, Pará, Brazil., de Queiroz Filho TN; Laboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas Institute, Belém, Pará, Brazil., da Cruz Carneiro AL; Laboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas Institute, Belém, Pará, Brazil., Falcão ASC; Department of Oral Pathology, School of Dentistry, Federal University of Para, Belém, Pará, Brazil., da Silva Kataoka MS; Department of Oral Pathology, School of Dentistry, Federal University of Para, Belém, Pará, Brazil., Pinheiro JJV; Department of Oral Pathology, School of Dentistry, Federal University of Para, Belém, Pará, Brazil., Rodrigues APD; Laboratory of Electron Microscopy, Section of Hepatology, Evandro Chagas Institute, Belém, Pará, Brazil. anarodrigues@iec.gov.br.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2023 Aug 16; Vol. 23 (1), pp. 223. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1186/s12866-023-02973-z
Abstrakt: Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. This study explores into the untapped territory of MMP-2 and MMP-9 within Leishmania spp. promastigotes. Notably, we successfully detected and quantified these MMPs, while also evaluating their activity in two distinct Leishmania species-L. amazonensis (La) and L. braziliensis (Lb)-at various growth stages and isolated from distinct clinical tegumentar disease forms. The results unveiled the presence of MMP-2 and MMP-9 in both species, albeit with distinct localization patterns. Specifically, MMP-9 exhibited significantly higher gelatinolytic activity in La when compared to Lb. Moreover, our data cleverly illustrated the presence and release of MMP-2 and MMP-9 by La and Lb promastigotes, exposing their ability to invade and migrate within a collagen matrix. This pioneering study establishes a compelling correlation between MMP-2 and MMP-9 and their potential role in the dynamics of La and Lb infection. Suggesting their potential as prognostic markers for severe leishmaniasis and promising target molecules for therapeutic interventions, this research opens new avenues for combatting this debilitating parasitic disease.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
Databáze: MEDLINE
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