Exposure to endocrine disruptors promotes biofilm formation and contributes to increased virulence of Pseudomonas aeruginosa.

Autor: Thiroux A; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Labanowski J; Université de Poitiers, UMR 7285, Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP), Poitiers, France., Venisse N; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France.; Université de Poitiers, CHU de Poitiers, INSERM, Centre d'investigation clinique CIC1402, Poitiers, France., Crapart S; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Boisgrollier C; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Linares C; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Berjeaud JM; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Villéger R; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France., Crépin A; Université de Poitiers, UMR CNRS 7267, Ecologie et Biologie des Interactions, Poitiers, France.
Jazyk: angličtina
Zdroj: Environmental microbiology reports [Environ Microbiol Rep] 2023 Dec; Vol. 15 (6), pp. 740-756. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1111/1758-2229.13190
Abstrakt: Anthropogenic activities contribute to the spread of chemicals considered as endocrine disruptors (ED) in freshwater ecosystems. While several studies have reported interactions of EDs with organisms in those ecosystems, very few have assessed the effect of these compounds on pathogenic bacteria. Here we have evaluated the impact of five EDs found in aquatic resources on the virulence of human pathogen P. aeruginosa. ED concentrations in French aquatic resources of bisphenol A (BPA), dibutyl phthalate (DBP), ethylparaben (EP), methylparaben (MP) and triclosan (TCS) at mean molar concentration were 1.13, 3.58, 0.53, 0.69, and 0.81 nM respectively. No impact on bacterial growth was observed at EDs highest tested concentration. Swimming motility of P. aeruginosa decreased to 28.4% when exposed to EP at 100 μM. Swarming motility increased, with MP at 1 nM, 10 and 100 μM (1.5-fold); conversely, a decrease of 78.5%, with DBP at 100 μM was observed. Furthermore, exposure to 1 nM BPA, DBP and EP increased biofilm formation. P. aeruginosa adhesion to lung cells was two-fold higher upon exposure to 1 nM EP. We demonstrate that ED exposure may simultaneously decrease mobility and increase cell adhesion and biofilm formation, which may promote colonisation and establishment of the pathogen.
(© 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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