| Autor: |
Wang Z; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Guo J; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Fan Q; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Tian Z; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Wang X; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Zheng W; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China., Huang L; College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China. |
| Abstrakt: |
Dracaena marginata is a widely cultivated horticultural plant in the world, which has high ornamental and medicinal value. In this study, the whole genome of leaves from D . marginata was sequenced by Illumina HiSeq 4000 platform. The chloroplast genome were assembled for functional annotation, sequence characteristics and phylogenetic analysis. The results showed that the chloroplast genome of D . marginata composed of four regions with a size of 154 926 bp, which was the smallest chloroplast genome reported for Dracaena species to date. A total of 132 genes were identified, including 86 coding genes, 38 tRNA genes and 8 rRNA genes. Codon bias analysis found that the codon usage bias was weak and there was a bias for using A/U base endings. 46 simple sequence repeat and 54 repeats loci were detected in the chloroplast genome, with the maximum detection rate in the large single copy region and inverted repeat region, respectively. The inverted repeats boundaries of D . marginata and Dracaena were highly conserved, whereas gene location differences occurred. Phylogenetic analysis revealed that D . serrulata and D . cinnabari form a monophyletic clade, which was the closest relationship and conformed to the morphological classification characteristics. The analysis of the chloroplast genome of D . marginata provides important data basis for species identification, genetic diversity and chloroplast genome engineering of Dracaena . |
