Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis ( Nasua nasua ) in periurban areas from Midwestern Brazil.

Autor:	Perles L; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, 14884-900, São Paulo, Brazil., Otranto D; Department of Veterinary Medicine, University of Bari, 70100, Valenzano, Italy.; Faculty of Veterinary Sciences, Bu-Ali Sina University, 6516738695, Hamedan, Iran., Barreto WTG; Post-Graduation of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande, 13471-410, Mato Grosso do Sul, Brazil., de Macedo GC; Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande, 13471-410, MS, Brazil., Lia RP; Department of Veterinary Medicine, University of Bari, 70100, Valenzano, Italy., Mendoza-Roldan JA; Department of Veterinary Medicine, University of Bari, 70100, Valenzano, Italy., Herrera HM; Post-Graduation of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande, 13471-410, Mato Grosso do Sul, Brazil.; Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande, 13471-410, MS, Brazil., de Oliveira CE; Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande, 13471-410, MS, Brazil., Machado RZ; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, 14884-900, São Paulo, Brazil., André MR; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, 14884-900, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	International journal for parasitology. Parasites and wildlife [Int J Parasitol Parasites Wildl] 2023 Aug 05; Vol. 22, pp. 14-19. Date of Electronic Publication: 2023 Aug 05 (Print Publication: 2023).
DOI:	10.1016/j.ijppaw.2023.08.002
Abstrakt:	Coatis ( Nasua nasua ) are wild carnivorous well adapted to anthropized environments especially important because they act as reservoirs hosts for many arthropod-borne zoonotic pathogens. Information about filarioids from coatis and associated Wolbachia spp. in Brazil is scant. To investigate the diversity of filarial nematodes, blood samples (n = 100 animals) were obtained from two urban areas in midwestern Brazil and analyzed using blood smears and buffy coats and cPCR assays based on the cox 1, 12S rRNA, 18S rRNA, hsp 70 and myo HC genes for nematodes and 16S rRNA for Wolbachia . When analyzing coati blood smears and buffy coats, 30% and 80% of the samples presented at least one microfilaria, respectively. Twenty-five cox 1 sequences were obtained showing 89% nucleotide identity with Mansonella ozzardi . Phylogenetic analyses clustered cox 1 sequences herein obtained within the Mansonella spp. clade. Sequences of both myo HC and two hsp 70 genes showed 99.8% nucleotide identity with Mansonella sp. and clustered into a clade within Mansonella sp., previously detected in coatis from Brazil. Two blood samples were positive for Wolbachia , with a 99% nucleotide identity with Wolbachia previously found in Mansonella perstans, Mansonella ozzardi and Mansonella atelensis and in ectoparasites of the genus Pseudolynchia, Melophagus and Cimex . The study showed a high prevalence of Mansonella sp. in the coati population examined, suggesting that this animal species play a role as reservoirs of a novel, yet to be described, species within the Onchocercidae family. Competing Interests: The authors declare no conflict of interest. (© 2023 The Authors.)
Databáze:	MEDLINE
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