Effects of passages through an insect or a plant on virulence and physiological properties of the fungus Metarhizium robertsii .

Autor:	Tomilova OG; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.; All-Russian Institute of Plant Protection, St. Petersburg, Russia., Kryukov VY; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia., Kryukova NA; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia., Tolokonnikova KP; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia., Tokarev YS; All-Russian Institute of Plant Protection, St. Petersburg, Russia., Rumiantseva AS; All-Russian Institute of Plant Protection, St. Petersburg, Russia., Alekseev AA; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russia., Glupov VV; Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Aug 11; Vol. 11, pp. e15726. Date of Electronic Publication: 2023 Aug 11 (Print Publication: 2023).
DOI:	10.7717/peerj.15726
Abstrakt:	Species of the genus Metarhizium are characterized by a multitrophic lifestyle of being arthropod parasites, rhizosphere colonizers, endophytes, and saprophytes. The process of adaptation to various organisms and substrates may lead to specific physiological alterations that can be elucidated by passaging through different hosts. Changes in virulence and cultivation properties of entomopathogenic fungi subcultured on different media or passaged through a live insect host are well known. Nevertheless, comparative in-depth physiological studies on fungi after passaging through insect or plant organisms are scarce. Here, virulence, plant colonization, hydrolytic enzymatic activities, toxin production, and antimicrobial action were compared between stable (nondegenerative) parent strain Metarhizium robertsii MB-1 and its reisolates obtained after eight passages through Galleria mellonella larvae or Solanum lycopersicum or after subculturing on the Sabouraud medium. The passaging through the insect caused similar physiological alterations relative to the plant-based passaging: elevation of destruxin A, B, and E production, a decrease in protease and lipase activities, and lowering of virulence toward G. mellonella and Leptinotarsa decemlineata as compared to the parent strain. The reisolates passaged through the insect or plant showed a slight trend toward increased tomato colonization and enhanced antagonistic action on tomato-associated bacterium Bacillus pumilus as compared to the parental strain. Meanwhile, the subculturing of MB-1 on the Sabouraud medium showed stability of the studied parameters, with minimal alterations relative to the parental strain. We propose that the fungal virulence factors are reprioritized during adaptation of M. robertsii to insects, plants, and media. Competing Interests: The authors declare that they have no competing interests. (© 2023 Tomilova et al.)
Databáze:	MEDLINE
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