Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells.

Autor: Buchanan E; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa., Mahony C; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa., Bam S; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa., Jaffer M; Electron Microscope Unit, University of Cape Town, Cape Town, 7700, South Africa., Macleod S; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa., Mangali A; Department of Physiological Sciences, Stellenbosch University, Matieland, Stellenbosch, 7602, South Africa., van der Watt M; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa., de Wet S; Department of Physiological Sciences, Stellenbosch University, Matieland, Stellenbosch, 7602, South Africa., Theart R; Department of Electrical and Electronic Engineering, Stellenbosch University, Matieland, Stellenbosch, 7602, South Africa., Jacobs C; Department of Pathology, Wellcome Centre for Infectious Diseases Research in Africa and IDM Microscopy Platform, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, 7700, South Africa., Loos B; Department of Physiological Sciences, Stellenbosch University, Matieland, Stellenbosch, 7602, South Africa., O'Ryan C; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7700, South Africa. colleen.oryan@uct.ac.za.; Neuroscience Institute, University of Cape Town, Cape Town, 7700, South Africa. colleen.oryan@uct.ac.za.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Aug 15; Vol. 13 (1), pp. 13248. Date of Electronic Publication: 2023 Aug 15.
DOI: 10.1038/s41598-023-40130-8
Abstrakt: Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (p < 0.01), Feret's diameter and perimeter (p < 0.05), and in area 2 (p < 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p < 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p < 0.0001), NRF1 (p < 0.01), TFAM (p < 0.05), STOML2 (p < 0.0001), and OPA1 (p < 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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