Proteomics Sequencing Reveals the Role of TGF-β Signaling Pathway in the Peripheral Blood of Offspring Rats Exposed to Fluoride.

Autor: Yu FF; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Yu SY; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Duan LZ; Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China., Yang S; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Hou XB; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Du YH; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Gao MH; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Zuo J; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Sun L; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Fu XL; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Li ZY; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Huang H; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Zhou GY; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China., Jia DL; Outpatient Department, Zhengyang County People's Hospital, Zhumadian, Henan, China., Chen RQ; Jinshui District Center for Disease Control and Prevention, Zhengzhou, Henan, China., Ba Y; Department of Environmental Health, School of Public Health, Environment and Health Innovation Team, Zhengzhou University, Zhengzhou, Henan, 450001, People's Republic of China. byyue@zzu.edu.cn.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2024 May; Vol. 202 (5), pp. 2100-2110. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1007/s12011-023-03805-x
Abstrakt: The underlying mechanism of fluorosis has not been fully elucidated. The purpose of this study was to explore the mechanism of fluorosis induced by sodium fluoride (NaF) using proteomics. Six offspring rats exposed to fluoride without dental fluorosis were defined as group A, 8 offspring rats without fluoride exposure were defined as control group B, and 6 offspring rats exposed to fluoride with dental fluorosis were defined as group C. Total proteins from the peripheral blood were extracted and then separated using liquid chromatography-tandem mass spectrometry. The identified criteria for differentially expressed proteins were fold change > 1.2 or < 0.83 and P < 0.05. Gene Ontology function annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the oeCloud tool. The 177 upregulated and 22 downregulated proteins were identified in the A + C vs. B group. KEGG pathway enrichment analysis revealed that transforming growth factor-β (TGF-β) signaling pathway significantly enriched. PPI network constructed using Cytoscape confirmed RhoA may play a crucial role. The KEGG results of genes associated with fluoride and genes associated with both fluoride and inflammation in the GeneCards database also showed that TGF-β signaling pathway was significantly enriched. The immunofluorescence in HPA database showed that the main expression sites of RhoA are plasma membrane and cytosol, while the main expression site of Fbn1 is the Golgi apparatus. In conclusion, long-term NaF intake may cause inflammatory response in the peripheral blood of rats by upregulating TGF-β signaling pathway, in which RhoA may play a key role.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: