Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice.

Autor: Tanihata J; Department of Cell Physiology, The Jikei University School of Medicine, Nishishinbashi, Minato-ku, Tokyo, Japan., Minamisawa S; Department of Cell Physiology, The Jikei University School of Medicine, Nishishinbashi, Minato-ku, Tokyo, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Aug 15; Vol. 18 (8), pp. e0289185. Date of Electronic Publication: 2023 Aug 15 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0289185
Abstrakt: Early detection of skeletal muscle atrophy is important to prevent further muscle weakness. However, there are few non-invasive biomarkers for skeletal muscle atrophy. Recent studies have reported that the N-terminal fragment (N-titin) of titin, a giant sarcomeric protein, is detected in the urine of patients with muscle damage. In this study, we hypothesized that urinary N-titin would be a potential early biomarker of skeletal muscle atrophy in mice caused by sciatic nerve denervation. Male mice were randomly divided into control and denervation groups, and urinary N-titin levels were assessed daily for 9 days using an enzyme-linked immunosorbent assay system. Despite reduced titin protein levels in atrophic muscles 10 days after denervation, cleaved N-titin fragments were not increased in the urine of mice with denervation-induced muscle atrophy. Furthermore, we found no uptake of Evans blue dye from the extracellular space into the cytoplasm in atrophic muscles, suggesting that the sarcomeric membrane is intact in those muscles. The present results suggest that cleaved N-titin in the urine is not suitable as an early biomarker of skeletal muscle atrophy.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Tanihata, Minamisawa. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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