Novel human lymph node-derived matrix supports the adhesion of metastatic oral carcinoma cells.

Autor: Naakka E; Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Translational Immunology Research Program (TRIMM), University of Helsinki, Helsinki, Finland., Wahbi W; Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Translational Immunology Research Program (TRIMM), University of Helsinki, Helsinki, Finland., Tiikkaja R; Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland.; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland., Juurikka K; Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland.; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland., Sandvik T; Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland.; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland., Koivunen P; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital, Oulu, Finland., Autio T; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital, Oulu, Finland., Tikanto J; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital, Oulu, Finland., Väisänen J; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital, Oulu, Finland., Tuominen H; Department of Pathology, Oulu University Hospital, Oulu, Finland., Talvensaari-Mattila A; Department of Obstetrics and Gynecology, Oulu University Hospital, Oulu, Finland., Al-Samadi A; Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Translational Immunology Research Program (TRIMM), University of Helsinki, Helsinki, Finland.; Institute of Dentistry, School of Medicine, University of Eastern Finland, Kuopio Campus, Kuopio, Finland., Soliymani R; Meilahti Clinical Proteomics Core Facility, Faculty of Medicine, HiLIFE, University of Helsinki, Helsinki, Finland., Åström P; Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland.; Biocenter Oulu, Oulu, Finland., Risteli M; Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland. maija.risteli@oulu.fi.; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland. maija.risteli@oulu.fi., Salo T; Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Translational Immunology Research Program (TRIMM), University of Helsinki, Helsinki, Finland.; Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland.; Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland.; Department of Pathology, HUSLAB, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland.
Jazyk: angličtina
Zdroj: BMC cancer [BMC Cancer] 2023 Aug 14; Vol. 23 (1), pp. 750. Date of Electronic Publication: 2023 Aug 14.
DOI: 10.1186/s12885-023-11275-6
Abstrakt: Background: 3D culture is increasingly used in cancer research, as it allows the growth of cells in an environment that mimics in vivo conditions. Metastases are the primary cause of morbidity and mortality in cancer patients, and solid tumour metastases are mostly located in lymph nodes. Currently, there are no techniques that model the pre-metastatic lymph node microenvironment in vitro. In this study, we prepared a novel extracellular matrix, Lymphogel, which is derived from lymph nodes, mimicking the tumour microenvironment (TME) of metastatic carcinoma cells. We tested the suitability of the new matrix in various functional experiments and compared the results with those obtained using existing matrices.
Methods: We used both commercial and patient-derived primary and metastatic oral tongue squamous cell carcinoma (OTSCC) cell lines. We characterized the functional differences of these cells using three different matrices (human uterine leiomyoma-derived Myogel, human pre-metastatic neck lymph node-derived Lymphogel (h-LG), porcine normal neck lymph node-derived Lymphogel (p-LG) in proliferation, adhesion, migration and invasion assays. We also performed proteomic analyses to compare the different matrices in relation to their functional properties.
Results: OTSCC cells exhibited different adhesion and invasion patterns depending on the matrix. Metastatic cell lines showed improved ability to adhere to h-LG, but the effects of the matrices on cell invasion fluctuated non-significantly between the cell lines. Proteomic analyses showed that the protein composition between matrices was highly variable; Myogel contained 618, p-LG 1823 and h-LG 1520 different proteins. The comparison of all three matrices revealed only 120 common proteins. Analysis of cellular pathways and processes associated with proteomes of each matrix revealed similarities of Myogel with h-LG but less with p-LG. Similarly, p-LG contained the least adhesion-related proteins compared with Myogel and h-LG. The highest number of unique adhesion-related proteins was present in h-LG.
Conclusions: We demonstrated that human pre-metastatic neck lymph node-derived matrix is suitable for studying metastatic OTSCC cells. As a whole-protein extract, h-LG provides new opportunities for in vitro carcinoma cell culture experiments.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
Databáze: MEDLINE
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