Light effects on Lasiodiplodia theobromae metabolome cultured in vitro.

Autor: Crispim AC; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil. alessandre89@gmail.com., Crispim SMA; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil., Rocha JR; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil., Ursulino JS; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil., Sobrinho RR; School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA., Porto VA; Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, AL, Brazil., Bento ES; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil., Santana AEG; Campus of Engineering and Agricultural Sciences, CECA Federal University of Alagoas, Maceió, AL, Brazil., Caetano LC; Chemistry and Biotechnology Institute, Federal University of Alagoas, Maceió, AL, Brazil.
Jazyk: angličtina
Zdroj: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2023 Aug 14; Vol. 19 (8), pp. 75. Date of Electronic Publication: 2023 Aug 14.
DOI: 10.1007/s11306-023-02041-7
Abstrakt: Introduction: The present work identified and compared intracellular metabolites and metabolic networks in mycelial cultures of Lasiodiplodia theobromae grown under 12 natural light and 24 hours' dark using a 1  H NMR-based metabolomics approach.
Materials and Methods: Fungal cultures were grown in potato dextrose media, and metabolites were extracted by sonication with sodium phosphate-buffered saline (pH = 6.0, 10% D 2 O, 0.1 mM TSP) from mycelium samples collected every week over four weeks.
Results: Multivariate analyses revealed that the light exposure group showed a positive correlation within beta-hydroxybutyrate, acetoacetate, acetone, betaine, choline, glycerol, and phosphocholine. On the other hand, phenyl acetate, leucine, isoleucine, valine, and tyrosine were positively correlated with dark conditions. Light favored the oxidative degradation of valine, leucine, and isoleucine, leading to the accumulation of choline, phosphocholine, betaine, and ketone bodies (ketogenesis). Ketogenesis, gluconeogenesis, and the biosynthesis of choline, phosphocholine, and betaine, were considered discriminatory routes for light conditions. The light-sensing pathways were interlinked with fungal development, as verified by the increased production of mycelia biomass without fruiting bodies and stress signaling, as demonstrated by the increased production of pigments.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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