Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response.

Autor:	Ruperti F; Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.; Collaboration for joint Ph.D. degree between EMBL and Heidelberg University, Faculty of Biosciences 69117 Heidelberg, Germany., Becher I; Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Stokkermans A; Hubrecht Institute, 3584 CT Utrecht, Netherlands., Wang L; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Marschlich N; Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.; Centre for Organismal Studies (COS), University of Heidelberg, 69120 Heidelberg, Germany., Potel C; Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Maus E; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Stein F; Proteomics Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Drotleff B; Metabolomics Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Schippers K; Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Nickel M; Bionic Consulting Dr. Michael Nickel, 71686 Remseck am Neckar, Germany., Prevedel R; Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Musser JM; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA., Savitski MM; Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.; Proteomics Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany., Arendt D; Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.; Centre for Organismal Studies (COS), University of Heidelberg, 69120 Heidelberg, Germany.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 02. Date of Electronic Publication: 2023 Aug 02.
DOI:	10.1101/2023.08.02.551666
Abstrakt:	A hallmark of animals is the coordination of whole-body movement. Neurons and muscles are central to this, yet coordinated movements also exist in sponges that lack these cell types. Sponges are sessile animals with a complex canal system for filter-feeding. They undergo whole-body movements resembling "contractions" that lead to canal closure and water expulsion. Here, we combine 3D optical coherence microscopy, pharmacology, and functional proteomics to elucidate anatomy, molecular physiology, and control of these movements. We find them driven by the relaxation of actomyosin stress fibers in epithelial canal cells, which leads to whole-body deflation via collapse of the incurrent and expansion of the excurrent system, controlled by an Akt/NO/PKG/A pathway. A concomitant increase in reactive oxygen species and secretion of proteinases and cytokines indicate an inflammation-like state reminiscent of vascular endothelial cells experiencing oscillatory shear stress. This suggests an ancient relaxant-inflammatory response of perturbed fluid-carrying systems in animals.
Databáze:	MEDLINE
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