Pathological, microscopic, and molecular diagnosis of paratuberculosis/John's disease in naturally infected dromedary camel ( Camelus dromedarius ).

Autor:	Tigani-Asil ETAE; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Abdelwahab GED; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Abdu EHAM; Extension Services and Animal Health Division, Animal Wealth Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Terab AMA; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Khalil NAH; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Marri ZJMA; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Yuosf MF; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Shah AAM; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Khalafalla AI; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates., Ishag HZA; Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.
Jazyk:	angličtina
Zdroj:	Veterinary world [Vet World] 2023 Jun; Vol. 16 (6), pp. 1277-1283. Date of Electronic Publication: 2023 Jun 08.
DOI:	10.14202/vetworld.2023.1277-1283
Abstrakt:	Background and Aim: Paratuberculosis (PTB) or John's disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed to highlight the anatomopathological changes of PTB in camels, which may help in verifying and validating some diagnostic tests used to detect the etiology of the disease in camel tissues. Materials and Methods: In August 2017, at Alselaa border's Veterinary Clinic of Al Dhafra Region, Western Abu Dhabi, UAE, one imported culled she-camel of 2 years old was subjected to clinical, microscopic, and anatomopathological investigations along with real-time quantitative polymerase chain reaction (q-PCR) to confirm the infection and correlate between clinical signs and pathological lesions of the PTB in dromedary camels. Results: Clinically, typical clinical signs compliant with the pathognomonic gross and histologic lesions of PTB were seen in naturally infected dromedary camel. As presumptive diagnosis microscopically, acid-fast coccobacillus bacterium clumps were demonstrated in direct fecal smears as well as in scraped mucosal and crushed mesenteric lymph node films, and in histopathological sections prepared from a necropsied animal and stained by Ziehl-Neelsen stain. Free and intracellular acid-fast clump phagosomes were further confirmed as Mycobacterium avium subsp. paratuberculosis by q-PCR. Conclusion: Clinical signs and pathological lesions of paratuberculosis in a dromedary camel were found to be similar to those of the other susceptible hosts. Competing Interests: The authors declare that they have no competing interests. (Copyright: © Tigani-Asil, et al.)
Databáze:	MEDLINE
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