Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response.

Autor: Miricescu A; Department of Biology Maynooth University Maynooth Ireland.; Pesticide Registration Division Department of Agriculture, Food and the Marine, Backweston Campus Celbridge Ireland., Brazel AJ; Department of Biology Maynooth University Maynooth Ireland., Beegan J; Smurfit Institute of Genetics Trinity College Dublin Dublin Ireland., Wellmer F; Smurfit Institute of Genetics Trinity College Dublin Dublin Ireland., Graciet E; Department of Biology Maynooth University Maynooth Ireland.; Kathleen Lonsdale Institute for Human Health Research Maynooth University Maynooth Ireland.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2023 Aug 12; Vol. 7 (8), pp. e518. Date of Electronic Publication: 2023 Aug 12 (Print Publication: 2023).
DOI: 10.1002/pld3.518
Abstrakt: Waterlogging leads to major crop losses globally, particularly for waterlogging-sensitive crops such as barley. Waterlogging reduces oxygen availability and results in additional stresses, leading to the activation of hypoxia and stress response pathways that promote plant survival. Although certain barley varieties have been shown to be more tolerant to waterlogging than others and some tolerance-related quantitative trait loci have been identified, the molecular mechanisms underlying this trait are mostly unknown. Transcriptomics approaches can provide very valuable information for our understanding of waterlogging tolerance. Here, we surveyed 21 barley varieties for the differential transcriptional activation of conserved hypoxia-response genes under waterlogging and selected five varieties with different levels of induction of core hypoxia-response genes. We further characterized their phenotypic response to waterlogging in terms of shoot and root traits. RNA sequencing to evaluate the genome-wide transcriptional responses to waterlogging of these selected varieties led to the identification of a set of 98 waterlogging-response genes common to the different datasets. Many of these genes are orthologs of the so-called "core hypoxia response genes," thus highlighting the conservation of plant responses to waterlogging. Hierarchical clustering analysis also identified groups of genes with intrinsic differential expression between varieties prior to waterlogging stress. These genes could constitute interesting candidates to study "predisposition" to waterlogging tolerance or sensitivity in barley.
Competing Interests: The authors declare that they have no conflicts of interest.
(© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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