Bioanalysis of MMR and KRAS - a key factor in diagnosis of colorectal cancer.

Autor:	Stefan-van Staden RI; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com +40213163113 +40751507779.; Faculty of Chemical Engineering and Biotechnologies, Politehnica University of Bucharest Bucharest Romania., Bratei AA; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com +40213163113 +40751507779.; Faculty of Chemical Engineering and Biotechnologies, Politehnica University of Bucharest Bucharest Romania.; Department of Pathology, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology Targu-Mures Romania., Ilie-Mihai RM; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com +40213163113 +40751507779., Gheorghe DC; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com +40213163113 +40751507779., Tuchiu BM; Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com +40213163113 +40751507779., Gurzu S; Department of Pathology, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology Targu-Mures Romania.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 Aug 11; Vol. 13 (34), pp. 24086-24092. Date of Electronic Publication: 2023 Aug 11 (Print Publication: 2023).
DOI:	10.1039/d3ra04260j
Abstrakt:	Two miniaturized electrochemical devices were designed for the simultaneous bioanalysis of MMR (MLH1, MSH2, MSH6, PMS2), and of KRAS in whole blood, urine, saliva, and tumoral tissues. The devices comprised besides the electronic part of the potentiostat a combined 3D stochastic microsensor (combined microplatform) with the sensing part based on the modification of graphene decorated with nitrogen, sulfur and boron (NSB-EGR) modified with two types of frutafit: FTEX and FHD. For the assay of MSH2, MSH6, KRAS, and PMS2 higher sensitivities were recorded when the microdevice based on FHD was used, while for the assay of MLH1 the best sensitivity was achieved by using the microdevice based on FTEX. While the limits of quantification for MLH1, MSH2, and PMS2 were not influenced by the modifier, the microdevice based on FHD provided the lowest limit of quantification for KRAS, the microdevice based on FTEX provided the lowest limit of quantification for MSH6. The validation tests performed proved that recoveries of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues higher than 98.50% with RSD (%) values lower than 0.10% were recorded. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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