Molecular detection of SARS-CoV-2 in domestic cats in close contact with positively-infected owners in Tehran, Iran in 2021.

Autor:	Saneei D; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Jamshidi S; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Ghalyanchi Langeroudi A; Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Akbarein H; Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Nadji SA; Virology Research Center, National Institutes of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Shoarzargari L; Virology Research Center, National Institutes of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Salehi-Vaziri M; Department of Arboviruses and Viral Hemorrhagic Fevers, Pasteur Institute of Iran, Tehran, Iran.; COVID-19 National Reference Laboratory, Pasteur Institute of Iran, Tehran, Iran., Moazezi Ghavihelm A; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Hojabr Rajeoni A; Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Shahbazi V; Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	JFMS open reports [JFMS Open Rep] 2023 Aug 10; Vol. 9 (2), pp. 20551169231172620. Date of Electronic Publication: 2023 Aug 10 (Print Publication: 2023).
DOI:	10.1177/20551169231172620
Abstrakt:	Objectives: In 2019, COVID-19 emerged in China and has since spread worldwide. Owing to the virus's ability to adhere to specific receptors, cats are susceptible to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The popularity of pet cats in Iran has sparked fears of human-cat-human transmission of the virus. This study aimed to identify positive cases in cats owned by people infected with SARS-CoV-2, to determine if they remained positive for >3 weeks and to examine the virus genome isolated from a number of cats and one of their owners. Methods: A total of 30 cats were sampled approximately 3 days after their owners tested positive (day 1), and 3 weeks later, in strict accordance with health regulations. Rectal and oropharyngeal samples were collected. All samples were subjected to a qualitative PCR and reverse transcription PCR. The S -gene region was partially sequenced in positive samples and the results were used to create a phylogenetic tree. Results: SARS-CoV-2 was detected in 7/30 (23.3%) cats examined. In the third week, every cat tested negative. The sequence data of positive cats and one of their owners revealed that the retrieved RNAs belonged to the alpha variation. The genetic distance between the samples and the reference sequence (20I/B.1.1.7: OM003849, MZ344997) was minimal, with a 99% similarity. Positive samples of cats had four mutations in gene S . Amino acid substitutions in the spike glycoprotein at positions N501Y, A570D, D614G and P681H were recorded in the isolates compared with 780 other sequences of Iranian strains. Conclusions and Relevance: This study confirmed the presence of SARS-CoV-2-infected cats living in close contact with infected owners. Despite cats' susceptibility to COVID-19, the risk of severe infection in these animals is low, as evidenced by the lack of clinical signs in positive cats. Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. (© The Author(s) 2023.)
Databáze:	MEDLINE
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