Behavioural and physiological responses of juvenile geoduck (Panopea zelandica) following acute thermal stress.

Autor: Sharma SS; Aquaculture Biotechnology Research Group, Department of Environmental Science, School of Science, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand., Venter L; Aquaculture Biotechnology Research Group, Department of Environmental Science, School of Science, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand., Frost EJ; Aquaculture Biotechnology Research Group, Department of Environmental Science, School of Science, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand., Alfaro AC; Aquaculture Biotechnology Research Group, Department of Environmental Science, School of Science, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand. Electronic address: andrea.alfaro@aut.ac.nz., Ragg NLC; Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand., Zamora LN; Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2024 Jan; Vol. 269, pp. 110892. Date of Electronic Publication: 2023 Aug 11.
DOI: 10.1016/j.cbpb.2023.110892
Abstrakt: Climate extremes, such as heatwaves, are expected to become more intense and of longer duration in the near future. These climatic conditions may have a significant impact on the prospects of establishing a new aquaculture industry for the endemic New Zealand geoduck, Panopea zelandica. This study focused on characterising animal behaviour, haemocytes , and heat shock protein (HSP70 & HSP90) mRNA expression following exposure to elevated temperatures, such as those encountered during marine heatwaves around 20 °C and an extreme scenario of 25 °C, contrasted to an ambient temperature of 17 °C. After 24 h of heat challenge, P. zelandica were found to be significantly influenced by the thermal changes, as there were differences recorded in all the responses examined. With increasing temperatures, juvenile geoduck were observed to fully emerge from the sediment a behaviour that has not previously been quantified nor associated with stress in this species. The ability of P. zelandica juveniles to re-bury still warrants further investigation, as adults are unable to do so. Haemocyte analyses revealed an increase in the abundance of granulocytes, cellular aggregations, and size of these aggregations at the highest temperature exposure. Increased expression of the hsp70 gene in the haemolymph after exposure at 25 °C for 24 h was detected and attributed to attempts to mitigate protein denaturation caused by thermal stress. The inter-individual variability in the response of heat shock proteins recorded could aid in future selective breeding programs if it is reflected in net thermotolerance. P. zelandica shows great potential for growing in subtidal habitats around New Zealand, and this study highlights the importance of temperature considerations when selecting potential farm and reseeding locations.
Competing Interests: Declaration of Competing Interest The Authors have no conflicts of interest to declare.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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