Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout.

Autor: Kulikouskaya V; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryna Str., 220084 Minsk, Belarus. Electronic address: kulikouskaya@gmail.com., Nikalaichuk V; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryna Str., 220084 Minsk, Belarus., Hileuskaya K; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryna Str., 220084 Minsk, Belarus., Ladutska A; Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, Belarus, 2 Kuprevich Str., 220084 Minsk, Belarus., Grigoryan K; Yerevan State University, 1 Alek Manukyan St, Yerevan 0025, Armenia., Kozerozhets I; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 31 Leninsky Prospect, 119991 Moscow, Russia., Hovsepyan V; Vanadzor State University, Tigran Mets 36, 2021 Vanadzor, Armenia., Sargsyan M; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 31 Leninsky Prospect, 119991 Moscow, Russia., Sidarenka A; Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, Belarus, 2 Kuprevich Str., 220084 Minsk, Belarus.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Nov 01; Vol. 251, pp. 126302. Date of Electronic Publication: 2023 Aug 12.
DOI: 10.1016/j.ijbiomac.2023.126302
Abstrakt: Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential <-50 mV) Alg-Ag NPs, which are stable during 9 months, included hot-plate assisted synthesis at 100 °C in diluted (1 mg/mL) Alg solutions. In vitro studies showed that Alg-Ag NPs exhibited prominent antimicrobial activity against collection Pseudomonas strains (inhibition zones ranged from 9.0 ± 1.0 to 19.0 ± 1.0 mm), with no significant loss of antibacterial efficacy after 9 months of storage. AFM analysis confirmed that the antibacterial effect of Alg-Ag NPs dealt with the direct nanomechanical disrupting of bacterial cells. The ability of Alg-Ag NPs to inhibit the growth of virulent P.aeruginosa, P.fluorescens and P. putida strains isolated from infected rainbow trout was evaluated. All tested strains were susceptible to Alg(10)-Ag NPs, while Alg(1)-Ag NPs demonstrated a limited strain-specific antibacterial effect. The obtained data displayed the prospects for the application of biogenic Alg-Ag NPs to create novel delivery systems for combating Pseudomonas infections in rainbow trout.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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