Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-specific memory B cells.

Autor: Krause RGE; Africa Health Research Institute, Durban, 4001, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa., Moyo-Gwete T; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Richardson SI; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Makhado Z; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Manamela NP; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Hermanus T; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Mkhize NN; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Keeton R; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Benede N; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Mennen M; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa., Skelem S; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa., Karim F; Africa Health Research Institute, Durban, 4001, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa., Khan K; Africa Health Research Institute, Durban, 4001, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa., Riou C; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa., Ntusi NAB; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa.; Hatter Institute for Cardiovascular Research in Africa, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa., Goga A; South African Medical Research Council, Cape Town, South Africa., Gray G; South African Medical Research Council, Cape Town, South Africa., Hanekom W; Africa Health Research Institute, Durban, 4001, South Africa.; Division of Infection and Immunity, University College London, London, WC1E 6BT, UK., Garrett N; Centre for the AIDS Program of Research in South Africa, Durban, South Africa.; Discipline of Public Health Medicine, School of Nursing and Public Health, University of KwaZulu-Natal, Durban, South Africa., Bekker LG; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Desmond Tutu HIV Centre, Cape Town, South Africa., Groll A; Department of Statistics, TU Dortmund University, Dortmund, Germany., Sigal A; Africa Health Research Institute, Durban, 4001, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa.; Centre for the AIDS Program of Research in South Africa, Durban, South Africa.; Max Planck Institute for Infection Biology, Berlin, 10117, Germany., Moore PL; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Centre for the AIDS Program of Research in South Africa, Durban, South Africa., Burgers WA; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa., Leslie A; Africa Health Research Institute, Durban, 4001, South Africa. al.leslie@ahri.org.; Division of Infection and Immunity, University College London, London, WC1E 6BT, UK. al.leslie@ahri.org.
Jazyk: angličtina
Zdroj: NPJ vaccines [NPJ Vaccines] 2023 Aug 12; Vol. 8 (1), pp. 119. Date of Electronic Publication: 2023 Aug 12.
DOI: 10.1038/s41541-023-00724-9
Abstrakt: Neutralizing antibodies strongly correlate with protection for COVID-19 vaccines, but the corresponding memory B cells that form to protect against future infection are relatively understudied. Here we examine the effect of prior SARS-CoV-2 infection on the magnitude and phenotype of the memory B cell response to single dose Johnson and Johnson (Ad26.COV2.S) vaccination in South African health care workers. Participants were either naïve to SARS-CoV-2 or had been infected before vaccination. SARS-CoV-2-specific memory B-cells expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a significant reduction in expression of the germinal center chemokine receptor CXCR5, and increased class switching. These B cell features correlated with neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 specific circulating T follicular helper cells (cTfh). Vaccination-induced effective neutralization of the D614G variant in both infected and naïve participants but boosted neutralizing antibodies against the Beta and Omicron variants only in participants with prior infection. In addition, the SARS-CoV-2 specific CD8+ T cell response correlated with increased memory B cell expression of the lung-homing receptor CXCR3, which was sustained in the previously infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the response to vaccination can provide insight into the impact of prior infection on memory B cell homing, CSM, cTfh, and neutralization activity. These data can provide early signals to inform studies of vaccine boosting, durability, and co-morbidities.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: