Comparison of Two Total Mercury Screening and Assessment Methods in Fishes: Biopsy Punch and Dried Muscle Samples.

Autor: Cyr A; Department of Veterinary Medicine, University of Alaska Fairbanks, 2141 Koyukuk Dr, Fairbanks, AK, 99775-7750, USA. acyr1@alaska.edu., Murillo-Cisneros DA; Centro de Investigaciones Biológicas Del Noroeste, S.C. Planeación Ambiental Y Conservación, Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico., López JA; University of Alaska Museum of the North, University of Alaska, 907 Yukon Drive, Fairbanks, AK, 99775-6960, USA.; College of Fisheries and Ocean Sciences, University of Alaska Fairbanks, 905 N Koyukuk Drive, Fairbanks, AK, 99775-7220, USA., Furin C; Alaska Department of Environmental Conservation, 5251 Dr. Martin Luther King Jr. Ave., Anchorage, AK, 99507, USA., O'Hara T; Bilingual Laboratory of Toxicology, Veterinary Integrative Biosciences, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Jazyk: angličtina
Zdroj: Archives of environmental contamination and toxicology [Arch Environ Contam Toxicol] 2023 Aug; Vol. 85 (2), pp. 119-128. Date of Electronic Publication: 2023 Aug 12.
DOI: 10.1007/s00244-023-01020-2
Abstrakt: Rapid and effective quantification of total mercury concentrations ([THg]) in fish muscle is an important part of ongoing monitoring to provide reliable and near real-time public health guidance. Methods for quantifying THg in fish muscle frequently require the use of large sample mass and numerous preparation steps. Wet (aka fresh weight) biopsy punch samples of fish muscle have been used to quantify THg directly, without drying and homogenization. Both methods have advantages and disadvantages. We compare the use of fresh weight biopsy punches for quantifying THg to using larger, dried homogenized samples. The [THg] determination for the two sampling methods was EPA method 7473. Three separate biopsy punch samples and a large muscle sample were taken from each fish and analyzed on a Direct Mercury Analyzer. There were no statistical differences between mean log transformed wet weight [THg] from biopsy punches and homogenized muscle across all samples or within individual species. Similarly, across the range of [THg] (7.5-612.7 ng/g ww), linear regression of [THg] from biopsy punch and homogenized muscle samples was not different from a 1:1 linear relationship. Linear regression statistics of [THg] with fish fork length produced similar results for both biopsy punch and homogenized muscle samples. However, the coefficient of variation among biopsy punch replicates for individual fish was frequently above the acceptable threshold of 15%. We recommend biopsy punches be used as an effective tool for broad-scale rapid monitoring of fish resources for Hg, while homogenized muscle samples be used for fine-scale ecological and health questions.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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