A reduced potential for lameness bacterial transmission by Lucilia sericata larvae and flies through metamorphosis.

Autor: Curwen AE; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK; School of Veterinary Medicine and Science, University of Nottingham, Leicestershire, UK., Still RL; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK., Angell JW; Wern Vets CYF, Department of Research and Innovation, Unit 11, Lon Parcwr Industrial Estate, Ruthin, Denbighshire LL15 1NJ, UK; Department of Livestock and One Health, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Neston CH64 7TE, UK., Eady PE; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK., Millson SH; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK., Staton GJ; Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Neston CH64 7TE, UK., Evans NJ; Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Neston CH64 7TE, UK., Duncan JS; Department of Livestock and One Health, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Neston CH64 7TE, UK., Cotter SC; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK., Clegg SR; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 8DB, UK; Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Neston CH64 7TE, UK. Electronic address: SClegg@Lincoln.ac.uk.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2023 Oct; Vol. 163, pp. 104967. Date of Electronic Publication: 2023 Aug 02.
DOI: 10.1016/j.rvsc.2023.104967
Abstrakt: Lameness in sheep is one of the most serious issues on farms in the UK and worldwide, affecting over 90% of all UK sheep flocks. Despite its severity and prevalence, there are knowledge gaps regarding transmission routes of bacterial pathogens associated with infectious lameness in sheep. As larvae of Lucilia sericata are commonly found on foot lesions on lame sheep, it was hypothesised that the flies or their larvae could harbour lameness associated bacteria. This study examined the gut contents of larvae obtained from the foot lesions of lame sheep and compared them to control larvae collected from infested cat food on the same farm. Of particular interest, were the presence of three different bacterial genera associated with lameness; Fusobacterium necrophorum, Dichelobacter nodosus and Treponema spp., for which viability was also investigated. Larvae were cultured In vitro and some allowed to metamorphose into flies before specific PCR assays were carried out on the gut contents. Results showed a significant association between the bacteria on the feet of the sheep and those within the larvae. Although the gut contents of all larvae found on sheep feet contained one or more of the lameness bacteria, none of the bacteria were recovered from the adult flies, suggesting a level of gut remodelling during metamorphosis. Interestingly, Treponema spp. and Fusobacterium spp. were viable when isolated from gut contents of larvae. Maintenance of infection from larvae to fly did not occur. However, it still remains important to control both disease and insect populations of farms to maintain animal welfare.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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