| Autor: |
Oishi K; Graduate School of Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Kobe 678-1297, Hyogo, Japan., Nagamori M; Graduate School of Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Kobe 678-1297, Hyogo, Japan., Kashino Y; Graduate School of Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Kobe 678-1297, Hyogo, Japan., Sekiguchi H; Center for Synchrotron Radiation Research, Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Sayo 679-5198, Hyogo, Japan., Sasaki YC; Center for Synchrotron Radiation Research, Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Sayo 679-5198, Hyogo, Japan.; Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa 277-8561, Chiba, Japan.; AIST-UTokyo Advanced Operando-Measurement Technology Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, 6-2-3 Kashiwanoha, Kashiwa 277-0882, Chiba, Japan., Miyazawa A; Graduate School of Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Kobe 678-1297, Hyogo, Japan., Nishino Y; Graduate School of Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Kobe 678-1297, Hyogo, Japan. |
| Abstrakt: |
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that play an important role in signal transduction at the neuromuscular junction (NMJ). Movement of the nAChR extracellular domain following agonist binding induces conformational changes in the extracellular domain, which in turn affects the transmembrane domain and opens the ion channel. It is known that the surrounding environment, such as the presence of specific lipids and proteins, affects nAChR function. Diffracted X-ray tracking (DXT) facilitates measurement of the intermolecular motions of receptors on the cell membranes of living cells, including all the components involved in receptor function. In this study, the intramolecular motion of the extracellular domain of native nAChR proteins in living myotube cells was analyzed using DXT for the first time. We revealed that the motion of the extracellular domain in the presence of an agonist (e.g., carbamylcholine, CCh) was restricted by an antagonist (i.e., alpha-bungarotoxin, BGT). |
